Substrate selectivity and its mechanistic insight of the photo-responsive non-nucleoside triphosphate for myosin and kinesin
Substrate selectivity and its mechanistic insight of the photo-responsive non-nucleoside triphosphate for myosin and kinesin
复制标题
光响应性非核苷三磷酸肌球蛋白和驱动蛋白的底物选择性及其机制
DOI：
10.1039/c8ob02714e
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发表时间：
2019
影响因子：
3.2
通讯作者：
N.
中科院分区：
文献类型：
--
作者：
Islam;Md. J.;Matsuo;K.;Menezes;H. M.;Takahashi;M.;Nakagawa;H.;Kakugo;A.;Sada;K.;Tamaoki;N.
线性马达蛋白包括驱动蛋白和肌球蛋白是开发纳米器件的有希望的生物材料。这些生物马达的光开关底物可用于在体外系统中光学调节其相关细胞骨架丝的运动性。在这里，我们描述了肌球蛋白选择性偶氮苯系三磷酸的发现。它使特定的光控制在一个可逆的模式与复合运动检测组成的驱动蛋白和肌球蛋白。这个肌球蛋白的选择性的机制的洞察力也解释了对接模拟研究。
Linear motor proteins including kinesin and myosin are promising biomaterials for developing nano-devices. Photoswitchable substrates of these biomotors can be used to optically regulate the motility of their associated cytoskeletal filaments in in vitro systems. Here, we describe the discovery of the myosin selective azobenzene-tethered triphosphate. It enables the specific photocontrol over myosin in a reversible mode with the composite motility assay composed of both kinesin and myosin. The mechanistic insight into this myosin selectivity is also explained with the docking simulation study.