Nestin expression in different tumours and its relevance to malignant grade
Nestin expression in different tumours and its relevance to malignant grade
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Nestin在不同肿瘤中的表达及其与恶性程度的相关性
DOI：
10.1136/jcp.2007.047605
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发表时间：
2008-04-01
影响因子：
3.4
通讯作者：
Xiang, A. P.
中科院分区：
文献类型：
--
作者：
Yang, X. H.;Wu, Q. L.;Xiang, A. P.
背景资料：巢蛋白是一种中间丝蛋白，在发育和再生组织的增殖祖细胞中表达，被鉴定为神经上皮前体细胞标志物。近年来，巢蛋白在神经胶质瘤、室管膜瘤、黑色素瘤、横纹肌肉瘤、胃肠道间质瘤和睾丸间质瘤中被发现。nestin的表达强度与胶质瘤的恶性程度相关。目的：检测不同肿瘤组织中nestin的表达，分析nestin表达与肿瘤恶性程度的关系。研究方法：肿瘤组织的福尔马林固定和石蜡包埋的手术标本来自中山大学病理学系。进行组织学分析和巢蛋白免疫组化染色。通过半定量评价分析组织学评分。结果如下：巢蛋白主要表达于血管肉瘤、胰腺癌和胃肠道间质瘤的细胞质中，部分肿瘤细胞表达于细胞核中。巢蛋白在高恶性GIST中的表达（2.2366（0.6920））与低恶性GIST中的表达（1.3783（0.4268））相比差异有统计学意义（p = 0.003），在高恶性血管肉瘤中的表达（1.9188（0.2069））与低恶性血管肉瘤中的表达（0.6474（0.3273））相比差异有统计学意义（p = 0.000）。 海绵状血管瘤不表达nestin。巢蛋白在高度恶性胰腺癌（7/14）中的组织学评分为1.1767（0.4676），在低度恶性胰腺癌（3/8）中的组织学评分为0.6577（0.0056），差异无统计学意义（P = 0.112）。 结论：结果提示，巢蛋白的表达可能在胃肠道间质瘤和血管肉瘤等肿瘤的发生发展中起重要作用。
Background: Nestin, an intermediate filament (IF) protein, is expressed in proliferating progenitor cells of developmental and regenerating tissues, and is identified as a neuroepithelial precursor cell marker. Recently, nestin was detected in some neoplasms such as glioma, ependymoma, melanoma, rhabdomyosarcoma, gastrointestinal stromal tumour (GIST), and testicular stromal tumour. Moreover, the expression intensity of nestin exhibited significant correlation with the malignant grade of glioma. Aims: To detect the expression of nestin in different tumours and to analyse the relationship between the expression of nestin and the malignant grade of the tumours. Methods: Formalin-fixed and paraffin-embedded surgical samples of neoplastic tissues were obtained from the Department of Pathology of Sun Yat-sen University. Histological analysis and immunohistochemical staining for nestin were performed. Histoscores were analysed by semi-quantitative evaluation. Results: Nestin was expressed predominantly in the cytoplasm of angiosarcoma, pancreatic adenocarcinoma and GIST samples, and some tumour cells expressed in the nucleus. There was a statistically significant difference between the histoscore of nestin in high malignant GIST (2.2366 (0.6920)) and that in low malignant GIST (1.3783 (0.4268)) (p = 0.003); and also between that in high malignant angiosarcoma (1.9188 (0.2069)) and that in low malignant angiosarcoma (0.6474 (0.3273)) (p = 0.000). Cavernous angioma did not express nestin. The histoscore of nestin in high malignant pancreatic adenocarcinoma (7/14) was 1.1767 (0.4676), and that in low malignant pancreatic adenocarcinoma (3/8) was 0.6577 (0.0056) (no significant difference, p = 0.112). Conclusions: Results suggest that the expression of nestin may play an important role in the development of some neoplasms such as GIST and angiosarcoma.