GROWTH-INHIBITION OF N1E-115 MOUSE NEUROBLASTOMA-CELLS BY C-MYC OR N-MYC ANTISENSE OLIGODEOXYNUCLEOTIDES CAUSES LIMITED DIFFERENTIATION BUT IS NOT COUPLED TO NEURITE FORMATION
GROWTH-INHIBITION OF N1E-115 MOUSE NEUROBLASTOMA-CELLS BY C-MYC OR N-MYC ANTISENSE OLIGODEOXYNUCLEOTIDES CAUSES LIMITED DIFFERENTIATION BUT IS NOT COUPLED TO NEURITE FORMATION
复制标题
DOI：
10.1016/0006-291x(92)91714-2
复制
发表时间：
1992-06-30
影响因子：
3.1
通讯作者：
GROS, F
中科院分区：
文献类型：
--
作者：
LARCHER, JC;BASSEVILLE, M;GROS, F
反义寡核苷酸在含胎牛血清的培养液(65℃加热灭活30min)和细胞中稳定存在。反义寡聚物处理会导致有丝分裂停止，但不会导致形态分化。在反义条件下，我们观察到两种神经特异性蛋白，即外周蛋白和伽马烯醇化酶的数量增加。比较化学诱导剂和反义寡核苷酸的结果，我们可以推测N1E-115分化的三个阶段：第一个阶段对应于有丝分裂的停止，第二个阶段对应于有限的神经元程序的表达，第三个阶段对应于形态和电生理的分化。
Antisense oligodeoxynucleotides were found to be stable in the culture medium containing fetal calf serum (heat-inactivated 30 minutes at 65°C) and in cells. Antisense oligomer treatment causes cessation of mitoses, but does not lead to morphological differentiation. Under antisense conditions, we have observed an increase in the amount of two neurospecific protein, namely peripherin and gamma-enolase. Comparison of the results obtained with chemical inducers and antisense olideoxynucleotides allows us to postulate three phases in N1E-115 differentiation: the first correspond to the arrest of mitosis, the second to the expression of a limited neuronal program, and the third to the morphological and electrophysiological differentiation.