Developmental myosin heavy chain progression in avian type IIB muscle fibres.
Developmental myosin heavy chain progression in avian type IIB muscle fibres.
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禽类 IIB 型肌纤维中肌球蛋白重链的发育进展。
DOI：
10.1007/bf01745118
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发表时间：
1991
影响因子：
2.7
通讯作者：
Yao,TT
中科院分区：
文献类型：
--
作者：
Rushbrook,JI;Weiss,C;Yao,TT
肌球蛋白重链物种进行了研究，在禽胸大肌（IIB型纤维）的发展过程中，通过高分辨率阴离子交换色谱的肌球蛋白头区，亚片段-1。在15天时，按照洗脱顺序，鉴别了4种不同的快型重链物质I、II、III和IV。到19 daysin卵，IV型已成为占主导地位，并通过3天后孵化仍然是主要的物种。此窗体已命名为theperihatch窗体。在孵化后3至5天之间，发生了第二次大规模的变化，孵化后5天，一种新的物种V占主导地位，在孵化后8天，V是唯一存在的重链物种。形式V，这对应于先前确定为theposthatchform，继续作为主要物种通过20天孵化后，慢慢地被成人形式所取代。来自三个亚片段-1重链物种（孵化前后（IV型）、孵化后（V型）和成虫）的溴化氰肽的N-末端测序显示氨基酸序列的差异与三种不同基因的产物一致。这些结果证实并扩展了最近关于鸡孵化前快速重链表达复杂性的报道（Hofmannet al.，1988;范霍恩和克劳，1989）。
Myosin heavy chain species were investigated during development in avian pectoralis major muscle (type IIB fibres) by high resolution anion-exchange chromatography of the myosin head region, subfragment-1. At 15 daysin ovofour distinct fast-type heavy chain species, I, II, III and IV, in order of elution, were identified. By 19 daysin ovo, form IV had become predominant and remained the major species through 3-days post-hatch. This form has been named theperihatch form. Between 3 and 5 days post-hatch, a second massive change occurred such that by 5 days post-hatch a new species, V, apparent at 19 daysin ovoin small amounts, dominated and at 8 days post-hatch was the only heavy chain species present. Form V, which corresponds to that previously identified as theposthatchform, continued as the major species through 20 days post-hatch and was replaced slowly by the adult form. N-terminal sequencing of CNBr peptides from three subfragment-1 heavy chain species, the peri-hatch (form IV), the post-hatch (form V) and adult, revealed differences in amino acid sequence consistent with the three being products of different genes. These results confirm and extend recent reports of complexity in fast heavy chain expression prior to hatching in the chicken (Hofmannet al., 1988; Van Horn & Crow, 1989).