Utilization of L-ascorbate by Escherichia coli K-12:: Assignments of functions to products of the yjf-sga and yia-sgb operons
Utilization of L-ascorbate by Escherichia coli K-12:: Assignments of functions to products of the yjf-sga and yia-sgb operons
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DOI：
10.1128/jb.184.1.302-306.2002
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发表时间：
2002-01-01
影响因子：
3.2
通讯作者：
Gerlt, JA
中科院分区：
文献类型：
--
作者：
Yew, WS;Gerlt, JA
大肠杆菌K-12能发酵L-抗坏血酸。在L-抗坏血酸（ula）的利用中编码分解代谢酶的操纵子已被鉴定;这种以前未知功能的操纵子已被指定为yif-sga操纵子。在产生D-木酮糖5-磷酸的途径中的三种酶已被功能性地表征：3-酮基-L-古洛糖酸6-磷酸脱羧酶（UIaD）、L-木酮糖5-磷酸3-差向异构酶（UlaE）和L-核酮糖5-磷酸4-差向异构酶（UlaF）。yia-sgb操纵子的几种产物也被功能性地表征，尽管操纵子的底物和生理功能仍然未知：2，3-二酮基-L-古洛糖酸还原酶（YiaK）、3-酮基-L-古洛糖酸激酶（LyxK）、3-酮基-L-古洛糖酸6-磷酸脱羧酶（SgbH）和L-核酮糖5-磷酸4-差向异构酶（SgbE）。
Escherichia coli K-12 can ferment L-ascorbate. The operon encoding catabolic enzymes in the utilization of L-ascorbate (ula) has been identified; this operon of previously unknown function had been designated the yif-sga operon. Three enzymes in the pathway that produce D-xylulose 5-phosphate have been functionally characterized: 3-keto-L-gulonate 6-phosphate decarboxylase (UIaD), L-xylulose 5-phosphate 3-epimerase (UlaE), and L-ribulose 5-phosphate 4-epimerase (UlaF). Several products of the yia-sgb operon were also functionally characterized, although the substrate and physiological function of the operon remain unknown: 2,3-diketo-L-gulonate reductase (YiaK), 3-keto-L-gulonate kinase (LyxK), 3-keto-L-gulonate 6-phosphate decarboxylase (SgbH), and L-ribulose 5-phosphate 4-epimerase (SgbE).