LINE-Like Retrotransposition in Saccharomyces cerevisiae
LINE-Like Retrotransposition in Saccharomyces cerevisiae
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DOI：
10.1534/genetics.108.096636
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发表时间：
2009-01-01
期刊：
影响因子：
3.3
通讯作者：
Han, Jeffrey S.
中科院分区：
文献类型：
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作者：
Dong, Chun;Poulter, Russell T.;Han, Jeffrey S.
超过三分之一的人类基因组序列是非 LTR 逆转录转座的产物。目前在人类中驱动这一过程的逆转录转座子非常丰富。 LINE-1 (L1) 元素。尽管 L1 在哺乳动物中普遍存在，但我们仍然缺乏对 L1 复制周期及其调控方式的完整机制理解。为了生成适合非 LTR 逆转录转座的遗传模型，我们重新设计了 Zorro3 逆转录转座子（白色念珠菌的 L1 同源物），用于芽殖酵母酿酒酵母。我们发现没有内源性L1同源物或残余物的酿酒酵母仍然可以支持Zorro3逆转录转座。对 Zorro3 突变体和插入结构的分析表明，这是真正的 L1 样逆转录转座，与哺乳动物 L1 介导的事件非常相似。这表明酿酒酵母出乎意料地保留了基础宿主。 L1 逆转座所需的机械。该模型还将作为一个强大的系统来研究 L1 元件的细胞生物学以及 L1 逆转录转座所涉及的细胞因子的遗传鉴定和表征。
Over one-third of human genome sequence is a product of non-LTR retrotransposition. The retrotransposon that currently drives this process in humans is the highly abundant. LINE-1 (L1) element. Despite the ubiquitous nature of L1's in mammals, we still lack a complete mechanistic understanding of the L1 replication cycle and how it is regulated. To generate a genetically amenable model for non-LTR retrotransposition, we have reengineered the Zorro3 retrotransposon, an L1 homolog from Candida albicans, for use in the budding yeast Saccharomyces cerevisiae. We found that S. cerevisiae, which has no endogenons L1 homologs or remnants, can still support Zorro3 retrotransposition. Analysis of Zorro3 mutants and insertion structures suggest that this is authentic L1-like retrotransposition with remarkable resemblance to mammalian L1-mediated events. This suggests that S. cerevisiae has unexpectedly retained the basal host. machinery required for L1 retrotransposition. This model will also serve as a powerful system to study the cell biology of L1 elements and for the genetic identification and characterization of cellular factors involved in L1 retrotransposition.