Structural Variation of the Superintegron in the Toxigenic Vibrio cholerae O1 El Tor
Structural Variation of the Superintegron in the Toxigenic Vibrio cholerae O1 El Tor
复制标题
产毒霍乱弧菌 O1 El Tor 中超级整合子的结构变异
DOI：
10.3967/0895-3988.2011.06.001
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发表时间：
2011-12-01
影响因子：
3.5
通讯作者：
Kan Biao
中科院分区：
文献类型：
--
作者：
Gao Yan;Pang Bo;Kan Biao
目的了解第七次霍乱大流行中霍乱弧菌超级整合子（SI）的遗传结构及变异情况。对1961 ~ 2008年分离的60株霍乱弧菌01 El Tor菌株进行分析。大多数缺失可能是霍乱弧菌重复序列之间重组的结果。大多数变异都聚集在一起。20世纪60年代和70年代分离的菌株的SI显示出更大的多样性，而20世纪90年代及以后分离的菌株的SI盒变异较低，具有类似于24 kb标签序列缺失。这表明在20世纪90年代及以后的流行期间，宿主中的主要SI。结论不同年代流行的霍乱弧菌SIs结构存在明显差异，而这些ElTor菌株SIs的同线结构差异是由于SIs的同线结构所致。此外，显示了第七次霍乱大流行期间宿主菌株SI中的持续盒流。
Objective To understand the genetic structures and variations of the superintegron (SI) in Vibrio cholerae isolated in the seventh cholera pandemic.Methods Polymerase chain reaction scanning and fragment sequencing were used. Sixty toxigenic V. cholerae 01 El Tor strains isolated between 1961 and 2008 were analyzed.Results Some variations were found, including insertions, replacements, and deletions. Most of the deletions were probably the result of recombination between V. cholerae repeat sequences. The majority of the variations clustered together. The SIs of the strains isolated in the 1960s and 1970s showed more diversity, whereas SI cassette variations in strains isolated in the 1990s and after were lower, with similar to 24 kb signature sequence deletion. This indicates the predominant SI in the host during the epidemic in the 1990s and after. The insertion cassettes suggested the mobilization from the SIs of other V. cholerae serogroups and Vibrio mimicus.Conclusion The study revealed that structural variations of SIs were obvious in the strains isolated in epidemics in different decades, whereas the divergence was based on syntenic structure of SIs in these El Tor strains. Also, the continuing cassette flows in the SIs of the host strains during the seventh cholera pandemics were displayed.