Capture of endogenously biotinylated proteins from Pseudomonas aeruginosa displays unexpected downregulation of LiuD upon iron nutrition.
Capture of endogenously biotinylated proteins from Pseudomonas aeruginosa displays unexpected downregulation of LiuD upon iron nutrition.
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从铜绿假单胞菌中捕获内源生物素化蛋白质显示出 LiuD 对铁营养的意外下调
DOI：
10.1016/j.bmc.2016.04.051
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发表时间：
2016
影响因子：
3.5
通讯作者：
Kaiser M
中科院分区：
文献类型：
--
作者：
Kaschani F;Dingemans J;van der Hoorn RA;Cornelis P;Kaiser M
过量铁的吸收和储存以及清除对微生物来说至关重要，因为过量的铁可能会导致活性氧物种的形成。因此，铁的动态平衡通常受到铁摄取调节器(Fur)的严格调控，Fur是一种全球性的铁调节因子，扮演着转录抑制因子的角色。在标记实验中检测生物素化蛋白时，我们发现内源性生物素化蛋白LiuD在铁处理后差异积累。LUD代表甲基巴豆酰-辅酶-羧基酶(MCCase)的α亚基，该酶来自亮氨酸/异戊酸利用途径。实时定量PCR转录分析显示，观察到的较低的LiuD生物素化水平可以追溯到较低的LiuD蛋白水平，这是通过转录抑制liuABCDE表达来实现的，但似乎不是由Fur介导的。根据LiuD在亮氨酸/异戊酸利用途径中的作用以及营养铁水平对其蛋白质水平的调节，我们发现，如果培养基中只含有亮氨酸作为碳源，野生型铜绿假单胞菌在铁的存在下不能生长。相反，当以葡萄糖为碳源时，铁促进生长。因此，我们的研究证明了铁调控的铜绿假单胞菌细菌生长的复杂性。
The uptake and storage but also removal of excess iron are of utmost importance to microorganisms since surplus levels of iron may lead to the formation of reactive oxygen species. Therefore, iron homeostasis is generally tightly regulated by the ferric uptake regulator (Fur), a global iron regulator acting as a transcriptional repressor. While detecting biotinylated proteins in labelling experiments, we discovered that the endogenously biotinylated protein LiuD differentially accumulated upon iron treatment. LiuD represents the α-subunit of the methylcrotonyl‐CoA‐carboxylase (MCCase), an enzyme from the leucine/isovalerate utilization pathway. Real-time PCR transcription analysis revealed that the observed lower levels of LiuD biotinylation could be traced back to lower LiuD protein levels via a transcriptional repression ofliuABCDEexpression that however does not seem to be mediated by Fur. In accordance with LiuD’s role for the leucine/isovalerate utilization pathway and its protein level regulation by nutritional iron levels, we found that wild-type Pseudomonas aeruginosa did not grow in the presence of iron if the medium contained only leucine as a carbon source. Conversely, iron stimulated the growth when glucose was used as a carbon source. Our study thus demonstrates the complexities of iron-regulated bacterial growth inPseudomonas aeruginosa.
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影响因子：
7
作者：
Joji Grace Villamor;F. Kaschani;T. Colby;Julian Oeljeklaus;David Zhao;M. Kaiser;M. Patricelli;R. V. D. van der Hoorn
通讯作者：
Joji Grace Villamor;F. Kaschani;T. Colby;Julian Oeljeklaus;David Zhao;M. Kaiser;M. Patricelli;R. V. D. van der Hoorn
DOI：
10.1073/pnas.0403423101
发表时间：
2004-06-29
影响因子：
11.1
作者：
Wilderman, PJ;Sowa, NA;Vasil, ML
通讯作者：
Vasil, ML
PvdS 的细胞内水平和活性，PvdS 是铜绿假单胞菌的主要铁饥饿西格玛因子
影响因子：
3.6
作者：
F. Tiburzi;F. Imperi;P. Visca
通讯作者：
P. Visca
影响因子：
7
作者：
Kaschani, Farnusch;Gu, Christian;van der Hoorn, Renier A. L.
通讯作者：
van der Hoorn, Renier A. L.
受感染植物的基于活性的蛋白质分析。
影响因子：
--
作者：
F. Kaschani;C. Gu;R. V. D. van der Hoorn
通讯作者：
R. V. D. van der Hoorn