DNA PLOIDY PROFILES AS PROGNOSTIC INDICATORS IN CIN LESIONS
DNA PLOIDY PROFILES AS PROGNOSTIC INDICATORS IN CIN LESIONS
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DOI：
10.1093/ajcp/92.3.261
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发表时间：
1989-09-01
影响因子：
3.5
通讯作者：
WIED, GL
中科院分区：
文献类型：
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作者：
BIBBO, M;DYTCH, HE;WIED, GL
探讨宫颈组织DNA倍体检测的预后意义。用基于个人计算机(PC)的视频显微测光系统对302例Feulgen染色的组织切片(91例正常鳞状上皮、14例尖锐湿疣、29例宫颈上皮内瘤变(CIN)I、78例CIN II和90例CIN III)进行了显微光度测量。对这些数据的分析表明，DNA图谱提供了重要的预后信息：具有多倍体DNA图谱的CIN比那些表现为非整倍体模式的CIN更有可能恢复正常。在211例异常病例中，38%为多倍体DNA图谱，62%为异倍体DNA图谱。消退的病例中86%是多倍体，14%是非整倍体。在130例非整倍体DNA病例中，95%的病例保持静止或进展，只有5%的病例消退。在这些不退化的非整倍体病变中，90个保持静止，34个进展，而在非退化多倍体组中，37个保持静止，只有6个进展。这一结果适用于不同的诊断类别。其他几个与倍体相关的指标也有预后意义(包括平均倍性、5N超标率和2N偏离指数，以及累积DNA直方图的顺序统计量分析得出的判别函数)，但没有达到DNA图谱分类的程度或表达的一致性。这些结果表明，通过Feulgen染色的组织标本的DNA图谱可以获得关于CIN病变预后的重要信息。这些数据是通过一个成本相对较低的细胞光度测量系统获得的，该系统由现成的硬件部件组成。
The prognostic significance of DNA ploidy measurements in cervical tissues was examined. Microphotometric measurements of 302 Feulgen-stained tissue sections (91 normal squamous epithelia, 14 condylomata, 29 cervical intraepithelial neoplasia (CIN) I, 78 CIN II, and 90 CIN III) were performed with a personal computer (PC)-based video microphotometry system. Analysis of these data shows that the DNA profile provides significant prognostic information: CINs with a polyploid DNA profile are more likely to return to normal than are those exhibiting an aneuploid pattern. Of 211 abnormal cases, 38% had polyploid DNA profiles and 62% were aneuploid. Eight-six percent of the cases that regressed were polyploid and 14% were aneuploid. Of the 130 aneuploid DNA cases, 95% remained static or progressed and only 5% regressed. Of these nonregressing aneuploid lesions, 90 remained static and 34 progressed, whereas within the nonregressing polyploid group 37 remained static and only 6 progressed. This result holds across diagnostic categories. Several other ploidy-related descriptors also showed prognostic significance (including mean ploidy, the 5N exceeding rate and 2N deviation index, and discriminant functions derived from order statistic analysis of the cumulative DNA histograms), but not to the degree or with the consistency of expression as the DNA profile categorization. These results indicate that important information about the prognosis of CIN lesions may be obtained by the DNA profile of Feulgen-stained tissue specimens. The data were acquired by a cytophotometry system of relatively modest cost consisting of readily available hardware components.