Penile erection induced in vivo by a purified toxin from the Brazilian spider Phoneutria nigriventer
Penile erection induced in vivo by a purified toxin from the Brazilian spider Phoneutria nigriventer
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DOI：
10.1111/j.1464-410x.2008.07762.x
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发表时间：
2008-10-01
影响因子：
4.5
通讯作者：
Srougi, Miguel
中科院分区：
文献类型：
--
作者：
Andrade, Enrico;Villanova, Fabiola;Srougi, Miguel
目的：观察黑腹拟声蜘蛛毒素Tx 2 -5和Tx 2 - 6对小鼠勃起功能的影响。称为“eretina”）直接注射到海绵体后，以评估效果所需的最小剂量。作用开始的时间、勃起的局部持续时间、组织学效应以及局部和全身副作用的存在。材料和方法当腹膜内应用时，eretina促进海绵体平滑肌的松弛，从而引起阴茎勃起。将35只小鼠分为两组; 10只对照小鼠注射20 μ L盐水溶液，在治疗组中，将25只小鼠分为5组，每个亚组接受递减剂量（0.024、0.012、0.006、0.003和0.0015 μ g/kg）的eretina，直至确定产生勃起的最小剂量。治疗后，监测211只小鼠以确定反应和任何附带效应。产生勃起的最小剂量为0.006 μ g/kg，该组中的5只小鼠在注射后35-45分钟有勃起的证据。用eretina处理的小鼠海绵体的组织学显示血管空间扩张和充血，比对照组有更多的血液。最小剂量没有局部或全身的副作用，120-140分钟后勃起消失。结论确定了eretina在小鼠中产生勃起的最小剂量。并且该试剂对于这种用途是安全的，因为它不产生任何附带的毒性作用。这些研究表明一种可能的手段，确定eretina的作用机制。
OBJECTIVESTo verify the effect on erectile tissue of mice of two neuropeptides extracted from the poison of a spider, Phoneutric nigriventer, (Tx2-5 and -6. termed 'eretina') after direct injection into the corpus cavernosum, to assess the minimum dosage necessary for effect. the time for initiation of action, the local duration of the erection, histological effects and the presence of local and systemic side-effects.MATERIALS AND METHODSWhen applied intraperitoneally, eretina promotes the relaxation of cavernous smooth muscle, thus causing penile erection. Thirty-five mice were divided in two groups; 10 control mice were injected 20 mu L of saline solution, and in the treated group, 25 mice were divided into groups of five and each subgroup received eretina in decreasing doses (0.024, 0.012, 0.006, 0.003 and 0.0015 mu g/kg) until the minimum dose that produced an erection was determined. After treatment 211 mice were monitored to determine the response and any collateral effects.RESULTSThe minimum dose producing an erection was 0.006 mu g/kg, the five mice in this group having evidence of an erection at 35-45 min after injection. The histology of the cavernosum of mice treated with eretina showed dilatation and congestion of the vascular spaces with more blood than in controls. With the minimum dose there were no local or systemic collateral effects and the erection was lost after 120-140 min.CONCLUSIONThe minimum dose of eretina producing an erection in mice was determined. and the agent was safe for this use as it did not produce any collateral toxic effects. These studies indicate a possible means of determining the mechanism of action of eretina.