Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry
Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry
复制标题
DOI：
10.1006/abio.1998.3077
复制
发表时间：
1999-04-10
影响因子：
2.9
通讯作者：
Reilly, JP
中科院分区：
文献类型：
--
作者：
Arnold, RJ;Reilly, JP
采用基质辅助激光解吸/电离飞行时间质谱法对大肠杆菌K-12菌株的核糖体进行了分析，具有良好的灵敏度和高质量准确度。56个亚基蛋白中的55个是可观察的。质谱峰。位置与以前报道的翻译后修饰一致，包括N-末端甲硫氨酸丢失、甲基化、硫甲基化和乙酰化，只有一个例外。质谱的快速性和准确性使其成为核糖体系统发育研究和其他生物体翻译后修饰观察的良好候选者。(C)北京：科学出版社.
Ribosomes from the K-12 strain of Escherichia coli were analyzed with good sensitivity and high mass accuracy using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Fifty-five of the 56 subunit proteins were observable. Mass spectral peak. locations were consistent with previously reported post-translational modifications involving N-terminal methionine loss, methylation, thiomethylation, and acetylation for all but one case. The speed and accuracy of mass spectrometry make it a good candidate for phylogenetic studies of ribosomes and the observation of posttranslational modifications in Other organisms. (C) 1999 Academic Press.