Characterization of a Cr51-labeled endotoxin and its identification in plasma and urine after parenteral administration.
Characterization of a Cr51-labeled endotoxin and its identification in plasma and urine after parenteral administration.
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肠胃外给药后CR51标记的内毒素的表征及其在血浆和尿液中的鉴定。
DOI：
10.1084/jem.117.4.561
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发表时间：
1963-04-01
影响因子：
15.3
通讯作者：
PARANT, M
中科院分区：
文献类型：
--
作者：
CHEDID, L;SKARNES, R C;PARANT, M
内毒素与Na2Cr51O4的孵育产生标记良好的产物。标记物固定在内毒素本身上，通过琼脂中形成的沉淀的特异性线上的放射自显影显示。在40，000RPM下的超离心沉降起始Boivin制剂总重量的80%。琼脂扩散模式与随后的放射自显影表明，铬标签仅与重馏分的颗粒。上清液含有可沉淀但未标记的内毒素。毒性测量表明，99%以上的总毒性存在于颗粒部分。在静脉注射LD 50或亚致死剂量后6小时内采集的血浆样品中特异性鉴别出铬酸盐标记的内毒素。内毒素在体内没有完全解毒，因为在肾上腺切除小鼠中测定时，即使是亚致死剂量注射后6小时收集的血浆也是有毒的。在尿液标本中特异性鉴定出内毒素，但其不再具有毒性或放射性。琼脂扩散实验表明，只有降解材料。
The incubation of endotoxin with Na2Cr51O4 yielded a product which was well labeled. That the label was fixed on the endotoxin itself was shown by autoradiography on specific lines of precipitation formed in agar. Ultracentrifugation at 40,000 RPM sedimented 80 per cent of the total weight of the starting Boivin preparation. Agar diffusion patterns with subsequent autoradiographs demonstrated that the chromium tag was associated only with the heavy fractions of the pellet. The supernatant contained precipitable, but unlabeled endotoxin. Toxicity measurements showed that more than 99 per cent of the total toxicity resided in the pellet fractions. The chromate-tagged endotoxin was specifically identified in plasma samples taken up to 6 hours after intravenous administration of LD 50 or sublethal doses. The endotoxin was not totally detoxified in vivo since plasma collected 6 hours after the injection of even the sublethal dose was toxic when assayed in adrenalectomized mice. The endotoxin was specifically identified in urine specimens but it was no longer toxic or radioactive. Agar diffusion experiments indicated that only degraded material was present.