Radioimmunoassay: a method for human chorionic gonadotropin and human luteinizing hormone.
Radioimmunoassay: a method for human chorionic gonadotropin and human luteinizing hormone.
复制标题
DOI：
10.1210/endo-79-1-10
复制
发表时间：
1966-07
期刊：
影响因子：
4.8
通讯作者：
A. Midgley
中科院分区：
文献类型：
--
作者：
A. Midgley
纯化的人绒毛膜促性腺激素已被放射性碘标记，以产生具有高比活性的免疫学和生物学活性产品。用这种标记的激素和能与人促黄体生成素和人绒毛膜促性腺激素特异性反应的抗血清，建立了灵敏和特异的放射免疫分析法。当通过放射免疫测定法和卵巢抗坏血酸消耗生物测定法估计在促黄体生成和促卵泡活性方面差异很大的制剂中促黄体生成激素的含量时，获得了可比的结果。该测定法灵敏度足以定量0.1 ml未浓缩血清和尿液中正常量的促黄体生成素。使用该测定法，在接近推测排卵的时间的妇女的血清和尿中发现促黄体生成激素浓度的峰值。（《内分泌学》79：10，1966）
Purified human chorionic gonadotropin has been radioiodinated to give an immunologically and biologically active product which possesses high specific activity. With this labeled hormone and an antiserum which is capable of reacting specifically with human luteinizing hormone and human chorionic gonadotropin, a sensitive and specific radioimmunoassay has been developed. Comparable results have been obtained when the content of luteinizing hormone in preparations differing widely in luteinizing and follicle stimulating activity have been estimated by the radioimmunoassay and the ovarian ascorbic acid depletion bio-assay. The assay is sufficiently sensitive to permit quantitation of normal amounts of luteinizing hormone in 0.1 ml of unconcentrated serum and urine. With the use of the assay, peaks in concentration of luteinizing hormone have been found in serum and urine of women near the time of presumed ovulation. (Endocrinology 79: 10, 1966)