QTL mapping of clubroot resistance in radish (Raphanus sativus L.)
QTL mapping of clubroot resistance in radish (Raphanus sativus L.)
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DOI：
10.1007/s00122-009-1230-z
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发表时间：
2010-03-01
影响因子：
5.4
通讯作者：
Hirai, Masashi
中科院分区：
文献类型：
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作者：
Kamei, Akito;Tsuro, Masato;Hirai, Masashi
以CR型日本萝卜与CS型中国萝卜杂交的F-2群体为材料，进行了萝卜抗根茎病（CR）的QTL分析。用F-2植株自交获得的F-3植株进行CR试验。接种盆栽苗，6周后评价症状。利用F-3植株的平均病害指数对F-2进行表型分析。分析两个CR试验结果是否存在QTL。利用AFLP和SSR标记构建连锁图谱；全长554 cM，包含18个连锁基团。两次检验均在连锁组1的顶端区域发现一个CR位点。因此，目前的结果表明，尽管不能排除其他基因组区域的轻微影响，但萝卜CR的很大一部分是由单个基因或紧密相连的基因控制的。CR位点命名为Crs1。与Crs1相关的标记与拟南芥3号染色体顶部的基因组区域序列同源，与油菜Crr3的情况相同。这些标记可用于萝卜CR品种的选育。由于已知日本萝卜对根茎病具有高度抗性或免疫，这些标记也可能有助于将CR基因渗入芸苔类作物。
A QTL analysis for clubroot resistance (CR) of radish was performed using an F-2 population derived from a crossing of a CR Japanese radish and a clubroot-susceptible (CS) Chinese radish. F-3 plants obtained by selfing of F-2 plants were used for the CR tests. The potted seedlings were inoculated and the symptom was evaluated 6 weeks thereafter. The mean disease indexes of the F-3 plants were used for the phenotype of the F-2. The results of two CR tests were analyzed for the presence of QTL. A linkage map was constructed using AFLP and SSR markers; it spanned 554 cM and contained 18 linkage groups. A CR locus was observed in the top region of linkage group 1 in two tests. Therefore, the present results suggest that a large part of radish CR is controlled by a single gene or closely linked genes in this radish population, although minor effects of other genomic areas cannot be ruled out. The CR locus was named Crs1. Markers linked to Crs1 showed sequence homology to the genomic region of the top of chromosome 3 of Arabidopsis, as in the case of Crr3, a CR locus in Brassica rapa. These markers should be useful for breeding CR cultivars of radish. As Japanese radishes are known to be highly resistant or immune to clubroot, these markers may also be useful in the introgression of this CR gene to Brassica crops.