Detection of fibrocytes in human skin wounds and its application for wound age determination
Detection of fibrocytes in human skin wounds and its application for wound age determination
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DOI：
10.1007/s00414-009-0320-4
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发表时间：
2009-07-01
影响因子：
2.1
通讯作者：
Kondo, Toshikazu
中科院分区：
文献类型：
--
作者：
Ishida, Yuko;Kimura, Akihiko;Kondo, Toshikazu
纤维细胞是一种新发现的细胞类型，是骨髓来源的间充质祖细胞，共表达造血细胞抗原和成纤维细胞产物。本研究采用抗cd45抗体和抗I型胶原抗体双色免疫荧光法检测53例不同创面年龄（I组，0-3天；II组，4-7天；III组，9-14天；IV组，17-21天）的人皮肤创面纤维细胞的时间依赖性外观。在年龄小于3天的伤口标本中，未检测到CD45(+)/胶原I型+纤维细胞。纤维细胞最初出现在创面4天，随着创面年龄的增加，纤维细胞的数量增加。半定量形态计量学分析显示，ⅲ组创面平均纤维细胞数最高。这些发现表明含有纤维细胞的人类皮肤伤口至少有4天的历史。此外，纤维细胞数量大于10表明伤口年龄在9 ~ 14天之间（即III组）。根据各组纤维细胞的平均数量，纤维细胞数量超过15个更强烈地表明伤口年龄为9-14天。总之，我们的观察结果表明，纤维细胞参与人体皮肤伤口愈合，诱导细胞外基质成分的积累，因此，纤维细胞的检测可能是确定伤口年龄的有用标记。
Fibrocytes, a newly identified cell type, are bone marrow-derived mesenchymal progenitors that coexpress hematopoietic cell antigens and fibroblast products. In this study, a double-color immunofluorescence analysis was carried out using anti-CD45 and anti-collagen type I antibodies to examine the time-dependent appearance of fibrocytes, using 53 human skin wounds with different wound ages (group I, 0-3 days; group II, 4-7 days; group III, 9-14 days; and group IV, 17-21 days). In wound specimens with an age of less than 3 days, CD45(+)/collagen type I+ fibrocytes were not detected. The fibrocytes were initially observed in wounds aged 4 days, and their number increased in lesions with advances in wound age. In a semiquantitative morphometrical analysis, the average number of fibrocytes was highest in the wounds of group III. These findings imply that human skin wounds containing fibrocytes are at least 4 days old. Moreover, a fibrocyte number of over 10 indicates a wound age between 9 and 14 days (i.e., group III). Based on the average number of fibrocytes in each group, a fibrocyte number of over 15 more strongly suggests a wound age of 9-14 days. Together, our observations indicate the participation of fibrocytes in wound healing of human skin inducing the accumulation of extracellular matrix components, and therefore, detection of fibrocytes could be a useful marker for wound age determination.