Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae
Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae
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DOI：
10.1083/jcb.200505020
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发表时间：
2005-10-24
影响因子：
7.8
通讯作者：
Dresser, ME
中科院分区：
文献类型：
--
作者：
Kateneva, AV;Konovchenko, AA;Dresser, ME
在第一次减数分裂时，姐妹染色单体凝聚力和同源重组相互协调，以促进同源染色体的分离，而不是姐妹染色单体的分离。在酿酒酵母减数分裂前期，减数分裂特异的粘附素Rec8p沿染色体轴定位，并介导大部分的凝聚力。有丝分裂粘附素Mcd1p/Scc1p定位于染色体臂上的离散点，其功能尚不清楚。在缺乏Tid1p的细胞中，Mcd1p和Rec8p在两次减数分裂中都异常存在，大多数细胞的染色体分离失败。Tid1p是参与染色质重建的解旋酶样蛋白SWI2/SNF2家族的成员。遗传学结果表明，这些细胞的主要缺陷是未能解决Mcd1p介导的连接。Tid1p与重组酶Dmc1p和Rad51p相互作用，在重组修复中具有既定的作用。我们认为Tid1p在减数分裂早期重塑Mcd1p介导的凝聚力，以促进同源重组和随后同源染色体的分离。
Sister chromatid cohesion and interhomologue recombination are coordinated to promote the segregation of homologous chromosomes instead of sister chromatids at the first meiotic division. During meiotic prophase in Saccharomyces cerevisiae, the meiosis-specific cohesin Rec8p localizes along chromosome axes and mediates most of the cohesion. The mitotic cohesin Mcd1p/Scc1p localizes to discrete spots along chromosome arms, and its function is not clear. In cells lacking Tid1p, which is a member of the SWI2/SNF2 family of helicase-like proteins that are involved in chromatin re-modeling, Mcd1p and Rec8p persist abnormally through both meiotic divisions, and chromosome segregation fails in the majority of cells. Genetic results indicate that the primary defect in these cells is a failure to resolve Mcd1p-mediated connections. Tid1p interacts with recombination enzymes Dmc1p and Rad51p and has an established role in recombination repair. We propose that Tid1p remodels Mcd1p-mediated cohesion early in meiotic prophase to facilitate interhomologue recombination and the subsequent segregation of homologous chromosomes.