The development of an ultra-sensitive electrochemical immunosensor using a PPyr-NHS functionalized disposable ITO sheet for the detection of interleukin 6 in real human serums
The development of an ultra-sensitive electrochemical immunosensor using a PPyr-NHS functionalized disposable ITO sheet for the detection of interleukin 6 in real human serums
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DOI：
10.1039/d0nj03183f
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发表时间：
2020-09-07
影响因子：
3.3
通讯作者：
Sezginturk, Mustafa Kemal
中科院分区：
文献类型：
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作者：
Aydin, Elif Burcu;Aydin, Muhammet;Sezginturk, Mustafa Kemal
以聚吡咯-N-羟基丁二酰亚胺（PPyr-NHS）聚合物为载体，制备了一种无标记阻抗免疫传感器，用于白细胞介素6（IL 6）抗原的超灵敏测定。在该传感平台中，PPyr-NHS聚合物首次被用作基质材料，该聚合物在其末端侧携带许多琥珀酰亚胺基团。该聚合物具有良好的生物相容性和导电性。此外，该材料作为基质材料的利用提供了IL 6受体（IL 6 R）的直接固定，IL 6受体（IL 6 R）被用作生物识别元件。用电化学阻抗谱（EIS）和循环伏安（CV）技术证实了工作电极的制备、IL 6 R的成功附着以及IL 6 R与IL 6抗原的特异性相互作用。在制作过程中，采用扫描电子显微镜（SEM）、原子力显微镜（AFM）、傅里叶变换红外光谱（FTIR）和拉曼光谱对工作电极表面进行了表征。采用阻抗法定量IL 6抗原。在优化的实验条件下，所设计的传感平台可检测IL 6抗原，检测范围为0.03 ~ 22.5 pg mL（-1），检测限为10.2 fg mL（-1）。此外，研制的生物传感器具有出色的灵敏度和特异性、良好的重复性和再现性、高稳定性和可重复使用性。此外，所设计的传感工具被成功地用于人类血清样品。因此，所建议的免疫传感器在临床上可用于通过简单稀释后直接测定血清IL 6抗原水平来早期检测前列腺癌。
A label-free impedimetric immunosensor based on a conjugated poly(pyrroleN-hydroxy succinimide) (PPyr-NHS) polymer covered disposable indium tin oxide electrode (ITO) was fabricated for the ultrasensitive determination of interleukin 6 (IL 6) antigen. In this sensing platform, the PPyr-NHS polymer, which carried a number of succinimide groups on its end side, was used as a matrix material for the first time. This synthesized polymer had excellent biocompatibility and good electrical conductivity. In addition, the utilization of this material as a matrix material provided direct immobilization of the IL 6 receptor (IL6R), which was employed as a biorecognition element. The preparation of the working electrode, the successful attachment of IL 6R and specific interaction between IL6R and IL 6 antigen were confirmed by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. Moreover, the working electrode surface was characterized by Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy during its construction. An impedimetric method was employed for the quantification of IL 6 antigen. Under optimized experimental conditions, the designed sensing platform could detect IL 6 antigen with a wide detection range from 0.03 pg mL(-1)to 22.5 pg mL(-1)with a relatively low detection limit of 10.2 fg mL(-1). In addition, the developed biosensor had outstanding sensitivity and specificity, good repeatability and reproducibility, high stability and reusability. Additionally, the designed sensing tool was successfully used in human serum samples. Consequently, the suggested immunosensor was clinically useful in the early detection of prostate cancer by direct determination of the serum IL 6 antigen level after simple dilution.