Methylation status of CEBPA gene promoter in chronic myeloid leukemia
Methylation status of CEBPA gene promoter in chronic myeloid leukemia
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DOI：
10.1179/1607845413y.0000000081
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发表时间：
2014-01-01
期刊：
影响因子：
1.9
通讯作者：
Battini, Mohan Reddy
中科院分区：
文献类型：
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作者：
Annamaneni, Sandhya;Kagita, Sailaja;Battini, Mohan Reddy
CCAAT/增强子结合蛋白α是髓细胞发育的关键转录因子之一，已被发现参与造血分化和白血病的发生。最近，通过DNA甲基化表达CEBPA的表观遗传调控已在白血病中得到证实。本研究旨在探讨慢性髓性白血病（CML）患者CEBPA基因的甲基化状态。采用甲基化特异性聚合酶链反应对来自印度海德拉巴的100例CML患者和98例正常健康人的CEBPA启动子甲基化状态进行了研究。100例CML病例中有32例发现CEBPA基因启动子甲基化异常。CEBPA基因启动子超甲基化频率与CML分期之间存在高度显著的相关性（P = 0.017），但与患者的年龄和性别没有相关性。结果表明，该基因启动子区CpG岛的异常甲基化可能是CML的常见事件，需要进行系统表达研究来揭示CEBPA启动子甲基化在CML的发生、进展和预后中的作用。
CCAAT/enhancer binding protein alpha is one of the crucial transcription factors for myeloid cell development that has been found to be involved in hematopoietic differentiation and leukemiogenesis. Recently, epigenetic regulation of CEBPA expression through DNA methylation has been demonstrated in leukemia. The aim of this study was to investigate the methylation status of CEBPA gene in chronic myeloid leukemia (CML) patients. The methylation status of CEBPA promoter was studied in 100 patients with CML and 98 normal healthy individuals from Hyderabad, India, using methylation-specific polymerase chain reaction. The aberrant methylation of CEBPA gene promoter was found in 32 of the 100 CML cases. A highly significant association was found between the frequency of CEBPA gene promoter hypermethylation and the CML stages (P = 0.017), but association with respect to age and gender of the patient was not found. The results suggest that aberrant methylation in the CpG island of the promoter region of this gene might be a common event in CML, and systemic expression studies will be needed to unfold the role of CEBPA promoter methylation in the development, progression, and prognosis of CML.