Overview of distinct 5-methylcytosine profiles of messenger RNA in human hepatocellular carcinoma and paired adjacent non-tumor tissues
Overview of distinct 5-methylcytosine profiles of messenger RNA in human hepatocellular carcinoma and paired adjacent non-tumor tissues
复制标题
人肝细胞癌和配对的邻近非肿瘤组织中信使 RNA 的独特 5-甲基胞嘧啶谱概述
DOI：
10.1186/s12967-020-02417-6
复制
发表时间：
2020-06-22
影响因子：
7.4
通讯作者：
Guo, Wenzhi
中科院分区：
文献类型：
--
作者：
Zhang, Qiyao;Zheng, Qingyuan;Guo, Wenzhi
研究背景转录后甲基化修饰，包括5-甲基胞嘧啶（5-methylcytosine，m5 C）修饰与肿瘤的发生密切相关。然而，在肝细胞癌（HCC）中m5 C修饰的mRNA谱是未知的。方法甲基化RNA免疫沉淀测序进行鉴定人HCC组织和癌旁组织的mRNA上的m5 C峰，并分析两组之间的差异m5 C。此外，我们进行了生物信息学分析，以预测特定的甲基化transcripts.ResultsWe的功能发现，有一个显着的差异，肝癌和配对的非肿瘤组织之间的m5 C，这表明，m5 C可以发挥作用，在肝癌的发病机制。此外，基因本体论和京都基因和基因组百科全书的分析表明，独特的分布模式的mRNA m5 C在HCC与广泛的细胞functions.ConclusionsOur结果显示不同的分布模式的m5 C在HCC和邻近组织，并提供了新的见解到一个新的功能的m5 C RNA甲基化的mRNA在HCC的进展。
BackgroundPost-transcriptional methylation modifications, including 5-methylcytosine (m5C) modification, are closely related to the tumorigenesis of cancers. However, the mRNA profile of m5C modification in hepatocellular carcinoma (HCC) is unknown.MethodsMethylated RNA immunoprecipitation sequencing was performed to identify m5C peaks on mRNA of human HCC tissues and adjacent tissues, and differences in m5C between the two groups were analyzed. In addition, we conducted a bioinformatics analysis to predict the function of specific methylated transcripts.ResultsWe found that there was a noticeable difference in m5C between HCC and paired non-tumor tissues, suggesting that m5C could play a role in the pathogenesis of HCC. In addition, analyses of gene ontology and the Kyoto Encyclopedia of Genes and Genomes showed that the unique distribution pattern of mRNA m5C in HCC was associated with a wide range of cellular functions.ConclusionsOur results revealed different distribution patterns of m5C in HCC and adjacent tissues and provided new insights into a novel function of m5C RNA methylation of mRNA in HCC progression.