Perspectives on polymeric gene delivery
Perspectives on polymeric gene delivery
复制标题
DOI：
10.1177/0883911503018002005
复制
发表时间：
2003-03-01
影响因子：
1.7
通讯作者：
Kabanov, AV
中科院分区：
文献类型：
--
作者：
Gebhart, CL;Kabanov, AV
非病毒基因递送领域的研究相对于更普遍已知的病毒系统处于初始阶段。然而，在不久的将来，非病毒系统可能会克服目前采用的病毒基因传递系统固有的一些问题。这些问题的范围从有限的有效载荷能力和一般生产问题到免疫和毒性反应，以及灾难性病毒重组的可能性。核酸和合成聚合物的自组装复合物，通常称为“聚合复合物”，是由于DNA的带负电荷的磷酸基团和聚阳离子的带正电荷的基团之间的静电相互作用而形成的。广泛的聚阳离子可用于此类研究，包括具有线性、支化、树枝状和嵌段或接枝共聚物结构的那些。这些聚阳离子在化学组成以及重复单元的数量上变化很大，提供了可以容易地组装的各种不同的聚合复合物。一些目前的基因传递系统的描述，作为潜在的试剂在该领域的聚合物为基础的基因传递。
Research in the field of nonviral gene delivery is in the initial stages relative to the more commonly known viral systems. However, nonviral systems may, in the near future overcome some of the problems inherent to currently employed viral gene delivery systems. These problems range from limited payload capacity and general production issues to immune and toxic reactions, as well as the potential for catastrophic viral recombination. Self-assembling complexes of nucleic acids and synthetic polymers, commonly referred to as 'polyplexes', are formed as the result of electrostatic interactions between the negatively charged phosphate groups of the DNA and the positively charged groups of the polycation. A wide array of polycations are available for such studies, including those with linear, branched, dendritic and block or graft copolymer architectures. These polycations vary greatly in chemical composition as well as the number of repeating units, providing for a wide range of different polyplexes that can be easily assembled. Some of the current gene delivery systems are described which serve as potential reagents in the field of polymer-based gene delivery.