INTERACTIONS AMONG REGULATORS OF RNA ABUNDANCE CHARACTERIZED USING RNA FINGERPRINTING BY ARBITRARILY PRIMED PCR
INTERACTIONS AMONG REGULATORS OF RNA ABUNDANCE CHARACTERIZED USING RNA FINGERPRINTING BY ARBITRARILY PRIMED PCR
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DOI：
10.1093/nar/22.21.4419
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发表时间：
1994-10-25
影响因子：
14.9
通讯作者：
WELSH, J
中科院分区：
文献类型：
--
作者：
MCCLELLAND, M;RALPH, D;WELSH, J
通过任意引物PCR使用RNA指纹，可以从匿名转录物的子集的协调行为推断会聚转录物调节途径，而无需克隆任何基因。可以估计每个响应类别中的转录本数量。对于差异显示也是如此。我们通过用两种已知的RNA丰度调节剂，转化生长因子-β（TGF β）和放线菌酮（CX），一起使用和单独使用来治疗细胞系来证明这些主张。超过1700个匿名成绩单的反应进行了监测，在这三种条件下，在未经处理的控制。在86个差异表达的转录本中观察到27个[3（3）]可能的转录本响应类别中的8个。例如，CX稳定或诱导多达2.7%的转录物，其中约三分之一在TGF β也存在时不积累。这种交叉可能反映了CX稳定或诱导一类重要的RNA，否则通常具有短的半衰期。我们预测，这类RNA构成了大多数针对TGF β和可能大多数其他天然转录调节剂的快速下调的转录物。
Using RNA fingerprinting by arbitrarily primed PCR it is possible to infer convergent transcript regulatory pathways from the coordinate behavior of subsets of anonymous transcripts without cloning any genes. The number of transcripts in each response category can be estimated. The same may be true for differential display. We demonstrate these claims by treating a cell line with two known modulators of RNA abundance, transforming growth factor-beta (TGF beta) and cycloheximide (CX), used together and alone. The responses of over 1700 anonymous transcripts were monitored under these three conditions and in an untreated control. Eight of the twenty-seven [3(3)] possible transcript response categories were observed among 86 differentially expressed transcripts. For example, CX stabilizes or induces as many as 2.7% of transcripts of which about one third do not accumulate when TGF beta is also present. This intersection may reflect CX stabilization or induction of an important class of RNAs that otherwise usually have short half-lives. We predict that RNAs in this class constitute the majority of transcripts targeted far rapid down regulation in response to TGF beta and perhaps most other natural transcriptional modulators.