Protein Signatures in Human MDA-MB-231 Breast Cancer Cells Indicating a More Invasive Phenotype Following Knockdown of Human Endometase/Matrilysin-2 by siRNA.
Protein Signatures in Human MDA-MB-231 Breast Cancer Cells Indicating a More Invasive Phenotype Following Knockdown of Human Endometase/Matrilysin-2 by siRNA.
复制标题
DOI：
10.7150/jca.2.165
复制
发表时间：
2011-03-16
影响因子：
3.9
通讯作者：
Sang QX
中科院分区：
文献类型：
--
作者：
Lee S;Terry D;Hurst DR;Welch DR;Sang QX
人基质金属蛋白酶-26（MMP-26/子宫内膜酶/基质溶解素-2）是乳腺癌、前列腺癌和其他上皮来源的癌症的推定生物标志物。在人乳腺癌细胞系MDA-MB-231中使用小干扰RNA（siRNA）沉默MMP-26表达。免疫学和蛋白质组学方法，包括二维凝胶电泳和基质辅助激光解吸/电离飞行时间质谱，用于确定MMP-26敲低细胞中的差异蛋白质表达。对照和MMP-26敲低细胞的蛋白表达谱的比较揭示了9个差异调节的蛋白。其中5种蛋白质（热休克蛋白90、葡萄糖调节蛋白78（GRP 78）、膜联蛋白V、原肌球蛋白和过氧化物氧还蛋白II）上调，而α-微管蛋白、半胱氨酸蛋白酶抑制剂SA-III、乳腺癌转移抑制因子1（BRMS 1）和β-肌动蛋白下调。BRMS 1表达的这种减少伴随着通过基质包被的膜的侵袭的增加。这些结果表明MMP-26在浸润性和转移性乳腺癌中参与蛋白质调节的重要作用。
Human matrix metalloproteinase-26 (MMP-26/endometase/matrilysin-2) is a putative biomarker for carcinomas of breast, prostate, and other cancers of epithelial origin. MMP-26 expression was silenced using small interfering RNA (siRNA) in the human breast cancer cell line MDA-MB-231. Immunological and proteomics approaches, including two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization time-of-flight mass spectrometry, were employed to identify differential protein expression in MMP-26 knockdown cells. A comparison of the protein expression profiles of control and MMP-26 knockdown cells revealed nine differentially regulated proteins. Five of the proteins (heat shock protein 90, glucose-regulated protein 78 (GRP78), annexin V, tropomyosin, and peroxiredoxin II) were up-regulated, while alpha-tubulin, cystatin SA-III, breast cancer metastasis suppressor 1 (BRMS1) and beta-actin were down-regulated. This decrease of BRMS1 expression is concomitant with an increase of invasion through matrix-coated membranes. These results suggest an important role for MMP-26 in the regulation of proteins involved in invasive and metastatic breast cancers.
登录
查看更多内容
影响因子：
6.4
作者：
Lehtonen, ST;Svensk, AM;Kinnula, VL
通讯作者：
Kinnula, VL
影响因子：
11.2
作者：
Li, W;Savinov, AY;Strongin, AY
通讯作者：
Strongin, AY
影响因子：
3.4
作者：
Hayashi, E;Kuramitsu, Y;Nakamura, K
通讯作者：
Nakamura, K
影响因子：
6.5
作者：
Ahokas, K;Skoog, T;Saarialho-Kere, U
通讯作者：
Saarialho-Kere, U
影响因子：
4.1
作者：
Lee, Seakwoo;Park, Hyun I.;Sang, Qing-Xiang Amy
通讯作者：
Sang, Qing-Xiang Amy