Protein half-lives of two subunits of an NMDA receptor-like complex, the 71-kDa glutamate-binding and the 80-kDa CPP-binding protein.
Protein half-lives of two subunits of an NMDA receptor-like complex, the 71-kDa glutamate-binding and the 80-kDa CPP-binding protein.
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NMDA 受体样复合物的两个亚基（71 kDa 谷氨酸结合蛋白和 80 kDa CPP 结合蛋白）的蛋白质半衰期。
DOI：
10.1006/bbrc.1997.7780
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Michaelis,EK
中科院分区：
文献类型：
--
作者：
Chen,X;Ferrington,DA;Bigelow,DJ;Michaelis,EK
我们确定了复合物的两个亚基的半衰期，该复合物在突触膜中充当谷氨酸和 N-甲基-D-天冬氨酸 (NMDA) 受体离子通道。这两种蛋白是 71 kDa 谷氨酸结合蛋白 (GBP) 和 80 kDa CPP 结合蛋白 (CBP)。 7 个月大的 Fischer 344 大鼠被注射 L-[14C] 亮氨酸。注射后 4 小时至 13 天处死的大鼠大脑中获得的粗制突触体膜组分测定了这两种蛋白质的放射性。先前报道的关于血清中 L-[14C] 亮氨酸放射性的时间依赖性外观和损失的数据（Ferrington 等，1997，Biochem.Biophys.Res.Commun.237, 163-165）在本研究中用于估计 GBP 和 CBP 的半衰期。假设 CBP 和 GBP 的表观半衰期分别为 14 (± 2.4) 和 18 (± 1.2) 小时，理论曲线最符合两种蛋白质获得的实验数据。
We determined the half-lives for two subunits of a complex that functions as a glutamate and N-methyl-D-aspartate (NMDA) receptor-ion channel in synaptic membranes. These two proteins are a 71 kDa glutamate-binding protein (GBP) and an 80 kDa CPP-binding protein (CBP). Seven month-old Fischer 344 rats were injected with L-[14C] leucine. The radioactivity in the two proteins was determined in a crude synaptosomal membrane fraction obtained from the brains of rats sacrificed from 4 hours to 13 days after the injection. The previously reported data on time-dependent appearance and loss of L-[14C] leucine radioactivity in the serum (Ferrington et al., 1997,Biochem. Biophys. Res. Commun.237, 163-165) was used in the present study to estimate the half-lives of GBP and CBP. Theoretical curves best fit the experimental data obtained for the two proteins assuming apparent half-lives of 14 (± 2.4) and 18 (± 1.2) hours for CBP and GBP, respectively.