The Up-regulation of Carbonic Anhydrase Genes of Bacillus mucilaginosus under Soluble Ca2+ Deficiency and the Heterologously Expressed Enzyme Promotes Calcite Dissolution
The Up-regulation of Carbonic Anhydrase Genes of Bacillus mucilaginosus under Soluble Ca2+ Deficiency and the Heterologously Expressed Enzyme Promotes Calcite Dissolution
复制标题
DOI：
10.1080/01490451.2014.884195
复制
发表时间：
2014-02
影响因子：
2.3
通讯作者：
Leilei Xiao;J. Hao;Weiying Wang;B. Lian;G. Shang;Yunwen Yang;Congqiang Liu;Shijie Wang
中科院分区：
文献类型：
--
作者：
Leilei Xiao;J. Hao;Weiying Wang;B. Lian;G. Shang;Yunwen Yang;Congqiang Liu;Shijie Wang
分子机制和基因调控是地质微生物学中研究微生物与矿物相互作用的重要领域。本文主要研究了碳酸氢酶(CA)基因在粘性芽孢杆菌中的表达调控，以及该基因在大肠杆菌中的表达产物对方解石风化的影响。采用实时荧光定量聚合酶链式反应(RT-qPCR)技术，探讨了在缺钙条件下粘质芽孢杆菌CA基因的表达与方解石溶解的关系。结果表明，在缺乏钙离子的培养基中加入方解石可以上调细菌CA基因的表达，从而加速方解石的溶解，有利于细菌的生长。用克隆的方法将粘质芽孢杆菌的CA基因导入大肠杆菌。然后，我们使用从产生的大肠杆菌菌株的粗酶提取物进行方解石溶解实验。酶提取物促进了方解石的溶解。这些发现为微生物CA在矿物风化和矿质营养释放中的作用提供了直接证据。
Molecular mechanisms and gene regulation are of interest in the area of geomicrobiology in which the interaction between microbes and minerals is studied. This paper focuses on the regulation of the expression of carbonic anhydrase (CA) genes in Bacillus mucilaginosus and the effects of the expression product of the B. mucilaginosus CA gene in Escherichia coli on calcite weathering. Real-time fluorescent quantitative PCR (RT-qPCR) was used to explore the relationship between CA gene expression in B. mucilaginosus and promotion of calcite dissolution under condition of Ca2+ deficiency. The results showed that adding calcite to the medium, which lacks Ca2+, can up-regulate the expression of the bacterial CA genes to accelerate calcite dissolution for bacterial growth. CA genes from B. mucilaginosus were transferred into E. coli by cloning. We then employed crude enzyme extract from the resultant E. coli strain in calcite dissolution experiments. The enzyme extract promoted calcite dissolution. These findings provide direct evidence for the role of microbial CA on mineral weathering and mineral nutrition release.