Desipramine induced changes in salivary proteins, cultivable oral microbiota and gingival health in aging female NIA Fischer 344 rats.
Desipramine induced changes in salivary proteins, cultivable oral microbiota and gingival health in aging female NIA Fischer 344 rats.
复制标题
地昔帕明诱导老年雌性 NIA Fischer 344 大鼠唾液蛋白、可培养口腔微生物群和牙龈健康的变化。
DOI：
10.1016/s0024-3205(00)00951-6
复制
发表时间：
2000
期刊：
影响因子：
6.1
通讯作者：
Humphreys-Beher,MG
中科院分区：
文献类型：
--
作者：
Koller,MM;Purushotham,KR;Maeda,N;Scarpace,PJ;Humphreys-Beher,MG
环状抗抑郁药仍然是用于治疗抑郁症的主要心理治疗药物。它们的主要副作用之一是唾液腺功能障碍（口腔干燥、口干症），导致人类口腔疾病增加以及言语、咀嚼、吞咽和味觉功能障碍。本研究的目的是评估长期服用三环类抗抑郁药地昔帕明对老年雌性 F344 大鼠特定唾液蛋白、口腔微生物群组成和口腔健康（牙龈炎）的影响，以及该治疗在 15 天冲洗期后的可逆性。唾液总蛋白浓度随着年龄和地昔帕明的增加而降低。相似的 SDS/PAGE 蛋白质谱出现在所有阶段，但随年龄和治疗的相对量不同。虽然某些蛋白质保持稳定水平（乳铁蛋白）或随着年龄和治疗而降低（淀粉酶），但地昔帕明和年龄会诱导富含脯氨酸的蛋白质、高分子量粘蛋白型糖蛋白和溶菌酶的合成。口腔微生物群随着年龄和抗抑郁药的使用而发生显着变化。地昔帕明引起牙龈炎的发病率在最年长的动物中最高。对于测量的不同参数，恢复随着年龄的增长而延迟。这些数据表明，地昔帕明对唾液蛋白分泌具有深远的影响。这可能部分解释了微生物群的变化和牙龈炎发病率的增加。
Cyclic antidepressants are still a dominating group of psychotherapeutic drugs used in the treatment of depression. One of their major side effect is salivary gland dysfunction (oral dryness, xerostomia), leading in humans to increased oral disease and dysfunction of speech, chewing, swallowing and taste. The purpose of this study was to assess the effects of the long-term administration of the tricyclic antidepressant desipramine and the reversibility of this treatment following a 15 d washout period on specific salivary proteins, composition of oral microbiota, and oral health (gingivitis) of aging female F344 rats. Total salivary proteins showed decreased concentrations with age and desipramine. Similar SDS/PAGE protein profiles appeared in all phases but in different relative amounts with age and treatment. While certain proteins maintained steady levels (lactoferrin) or decreased with age and treatment (amylase), the synthesis of proline-rich proteins, high molecular weight mucin-type glycoproteins, and lysozyme was induced with desipramine and age. The oral microbiota was significantly changed with age and the administration of the antidepressant. The incidence of gingivitis with desipramine was highest in the oldest animals. For the different parameters measured, recovery was delayed with age. These data indicate, that desipramine has profound effects on salivary protein secretion. This may partially explain the changes in microbiota and the increased incidence of gingivitis.