Cycloheximide-resistant temperature-sensitive lethal mutations of Saccharomyces cerevisiae.
Cycloheximide-resistant temperature-sensitive lethal mutations of Saccharomyces cerevisiae.
复制标题
酿酒酵母的放线菌酮抗性温度敏感致死突变。
DOI：
10.1093/genetics/119.2.303
复制
发表时间：
1988
期刊：
影响因子：
3.3
通讯作者：
Haber,JE
中科院分区：
文献类型：
--
作者：
McCusker,JH;Haber,JE
我们描述了一组多效性突变的分离和初步表征耐放线菌酮的最低抑制浓度和筛选TS（温度敏感）的增长。这些突变属于放线菌酮抗性ts致死突变（crl）的22个互补组。crl突变似乎与先前分离的突变无关。15个CRL基因座已被定位。在非允许温度（37度）下，这些突变体在几次细胞分裂后在细胞周期后期停止。这些突变体中有一半也无法在非常低的温度（5度）下生长。尽管来自所有22个互补组的突变体表现出相似的温度敏感表型，crl3的ts致死性的基因外抑制子并不能减轻大多数其他crl突变体的ts致死性。第二个抑制突变允许crl10，crl12和crl14在37度生长，但不抑制其余crl突变体的ts致死性。我们还描述了两种新的方法，用于从野生型酵母菌株中富集营养缺陷型突变。
We describe the isolation and preliminary characterization of a set of pleiotropic mutations resistant to the minimum inhibitory concentration of cycloheximide and screened for ts (temperature-sensitive) growth. These mutations fall into 22 complementation groups of cycloheximide resistant ts lethal mutations (crl). None of the crl mutations appears to be allelic with previously isolated mutations. Fifteen of the CRL loci have been mapped. At the nonpermissive temperature (37 degrees), these mutants arrest late in the cell cycle after several cell divisions. Half of these mutants are also unable to grow at very low temperatures (5 degrees). Although mutants from all of the 22 complementation groups exhibit similar temperature-sensitive phenotypes, an extragenic suppressor of the ts lethality of crl3 does not relieve the ts lethality of most other crl mutants. A second suppressor mutation allows crl10, crl12, and crl14 to grow at 37 degrees but does not suppress the ts lethality of the remaining crl mutants. We also describe two new methods for the enrichment of auxotrophic mutations from a wild-type yeast strain.