LUMINOL-ENHANCED CHEMILUMINESCENCE AFTER REACTION OF HYDROPEROXIDES WITH OPSONIZED ZYMOSAN
LUMINOL-ENHANCED CHEMILUMINESCENCE AFTER REACTION OF HYDROPEROXIDES WITH OPSONIZED ZYMOSAN
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DOI：
10.1002/bio.1170100202
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发表时间：
1995-03-01
期刊：
影响因子：
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通讯作者：
ELDESSOUKI, J
中科院分区：
文献类型：
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作者：
HEINLE, H;ELDESSOUKI, J
鲁米诺增强化学发光（LEG）在检测自由基方面非常敏感，但对氢过氧化物（过氧化氢、叔丁基过氧化氢）相对不敏感。然而，在调理酵母聚糖（通常用于刺激吞噬细胞）的存在下，过氧化氢也诱导LEG，表明在这些条件下产生自由基。因此，当LEC用于表征酵母多糖诱导的吞噬作用时，仔细解释反应性物质的性质是必要的。本文研究了酵母多糖在不同条件下诱导的LEC的性质，并与各种抑制剂进行了比较。典型的自由基清除剂，如去甲二氢愈创木酸和超氧化物歧化酶，是强抑制剂，表明超氧阴离子的重要性。该系统可用于抗氧化或自由基清除活性方面的药物测试。
Luminol-enhanced chemiluminescence (LEG) is very sensitive in detecting free radicals but relatively insensitive for hydroperoxides (hydrogen peroxide, tert-butyl hydroperoxide). However, in the presence of opsonized zymosan (often used for stimulation of phagocytic cells) hydroperoxides also induce LEG, suggesting that free radicals are produced under these conditions. Therefore careful interpretation with respect to the nature of the reactive species is necessary when LEC is used for characterization of zymosan-induced phagocytosis. We studied the properties of zymosan-induced LEC under different test conditions and with various inhibitors, Typical radical scavengers, e.g. nordihydroguaiaretic acid and superoxide dismutase, are strong inhibitors, indicating the importance of the superoxide anion. This system is useful for drug testing with respect to antioxidative or radical scavenging activity.