CHARACTERIZATION OF DRUG IONTOPHORESIS WITH A FAST MICROASSAY TECHNIQUE
CHARACTERIZATION OF DRUG IONTOPHORESIS WITH A FAST MICROASSAY TECHNIQUE
复制标题
DOI：
10.1016/s0006-3495(76)85723-2
复制
发表时间：
1976-01-01
影响因子：
3.4
通讯作者：
DIONNE, VE
中科院分区：
文献类型：
--
作者：
DIONNE, VE
使用离子选择性微电极测定方法描述了药物从微量移液器到游离（林格氏）溶液的离子电渗释放。该表征的时间分辨率为 20 ms，表明药物转运的平衡速率与释放电流不成线性比例；后备电流增加了对线性的偏离，并用分析得出的药物释放函数证明了结果。药物转运速率和释放电流之间的一般关系与特定药物或移液器阻力无关；没有观察到可以在不事先使用测定的情况下定量预测这种依赖性的函数关系。 25° 时的扩散系数测定本研究中使用的药物（所有神经肌肉激动剂）的青蛙林格氏C：所有值×。 106平方厘米/秒；乙酰胆碱 6.11.+-. 0.30；氨甲酰胆碱 7.44 .+-. 0.34； 3-(间羟基苯基)丙基三甲基铵5.79.+-。 0.13。
The iontophoretic release of drugs from micropipettes into free (Ringer''s) solution was described using an ion-selective microelectrode assay method. This characterization, with a temporal resolution of 20 ms, showed that the equilibrium rate of drug transport was not linearly proportional to release current; the departure from linearity was increased by backing current and the result was demonstrated with analytically derived drug release functions. The general relation between the drug transport rate and release current was independent of the specific drug or pipette resistance; no functional relation was observed that might quantitatively predict this dependence without prior use of the assay. The diffusion coefficients at 25.degree. C in frog Ringer''s of the drugs used in this study, all neuromuscular agonists, were determined: all values .times. 106 cm2/s; acetylcholine 6.11 .+-. 0.30; carbamylcholine 7.44 .+-. 0.34; 3-(m-hydroxyphenyl) propyltrimethyl ammonium 5.79 .+-. 0.13.