Mapping an endometrial cancer tumor suppressor gene at 10q25 and development of a bacterial clone contig for the consensus deletion interval.
Mapping an endometrial cancer tumor suppressor gene at 10q25 and development of a bacterial clone contig for the consensus deletion interval.
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DOI：
10.1006/geno.1998.5399
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发表时间：
1998-08
期刊：
影响因子：
4.4
通讯作者：
Stacia Peiffer-Schneider;F. Noonan;D. Mutch;Sally B. Simpkins;T. Herzog;J. Rader;A. Elbendary;D. Gersell;K. Call;P. Goodfellow
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文献类型：
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作者：
Stacia Peiffer-Schneider;F. Noonan;D. Mutch;Sally B. Simpkins;T. Herzog;J. Rader;A. Elbendary;D. Gersell;K. Call;P. Goodfellow
子宫内膜癌中染色体10 q序列的频繁丢失提示肿瘤抑制基因的参与。先前的杂合性丢失（洛）研究指出10 q25-q26区域可能是子宫内膜肿瘤发生中的肿瘤抑制基因（S. L. Peiffer等人，1995，Cancer Res. 55：1922-1926; S. Nagase等人，1996年，Br. J. Cancer 74：1979-1983; S. Nagase等人，57：1630-1633）。为了进一步明确10 q25的抑癌基因定位，我们筛选了123例乳腺样腺癌10q25.3序列杂合性缺失。43例（35%）在一个或多个位点发现洛缺失。观察到的等位基因丢失模式定义了D10 S221和D10 S610之间的最小共有缺失区域。作为发现10 q25肿瘤抑制基因实验的第一步，开发了一个序列准备好的细菌克隆重叠群和一个跨越缺失区的1 Mb间隔的长距离限制性内切酶图谱。
Frequent loss of chromosome 10q sequences in endometrial cancers suggests the involvement of a tumor suppressor gene. Previous loss-of-heterozygosity (LOH)studies have pointed to the 10q25-q26 region as the likely site of a tumor suppressor involved in endometrial tumorigenesis (S. L. Peiffer et al., 1995, Cancer Res. 55: 1922-1926; S. Nagase et al., 1996, Br. J. Cancer 74: 1979-1983; S. Nagase et al.,1997, Cancer Res. 57: 1630-1633). In an attempt to define further the localization of a tumor suppressor gene at 10q25, we screened a panel of 123 endometrioid adenocarcinomas for loss of heterozygosity of 10q25.3 sequences. Forty-three (35%) revealed LOH at one or more loci. The observed patterns of allelic loss define a minimum consensus region of deletion between D10S221 and D10S610. A sequence-ready bacterial clone contig and a long-range restriction map for a 1-Mb interval spanning the deletion region were developed as the first step in experiments directed toward the discovery the 10q25 tumor suppressor.