Improved in vitro efficacy of gold nanoconstructs by increased loading of G-quadruplex aptamer.
Improved in vitro efficacy of gold nanoconstructs by increased loading of G-quadruplex aptamer.
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DOI：
10.1021/nl500844m
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发表时间：
2014-05-14
期刊：
影响因子：
10.8
通讯作者：
Odom TW
中科院分区：
文献类型：
--
作者：
Dam DH;Lee RC;Odom TW
本文描述了如何在体外的适配体加载的金纳米星（Apt-AuNS）的效力可以通过增加加载的G-四链体同源二聚体AS1411（Apt）的AuNS表面上增强。在低pH缓冲环境中，Apt在AuNS上的负载密度增加到使用常规盐老化过程获得的2.5倍。这些高度负载的AuNS纳米构建体（* Apt-AuNS）在胰腺癌和纤维肉瘤细胞ca.与Apt-AuNS相比，速度快2倍。当癌细胞内化相似数量的AuNS载体时，通过 * Apt-AuNS递送的AS1411的量是Apt-AuNS的两倍，并且 * Apt-AuNS导致细胞死亡平均增加42%。这些结果表明，增加AuNS上的负载密度可以提供一种简单的方法来改善纳米构建体在癌细胞中的摄取以及体外功效。
This paper describes how in vitro efficacy of aptamer-loaded gold nanostars (Apt-AuNS) can be enhanced by the increased loading of a G-quadruplex homodimer AS1411 (Apt) on the AuNS surface. In a low pH buffer environment, the loading density of Apt on AuNS was increased up to 2.5 times that obtained using the conventional salt-aging process. These highly loaded AuNS nanoconstructs (*Apt-AuNS) were taken up in pancreatic cancer and fibrosarcoma cells ca. 2 times more and at faster rates compared to Apt-AuNS. When a similar number of AuNS carriers was internalized by the cancer cells, the amount of AS1411 delivered via *Apt-AuNS was effectively double that of Apt-AuNS, and *Apt-AuNS resulted in an average of 42% increase in cell death. These results suggest that increasing the loading density on AuNS could provide a simple means to improve uptake as well as in vitro efficacy of the nanoconstructs in cancer cells.
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