Culturing and Neuronal Differentiation of Human Dental Pulp Stem Cells.
Culturing and Neuronal Differentiation of Human Dental Pulp Stem Cells.
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DOI：
10.1002/cphg.28
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发表时间：
2017-01-11
影响因子：
--
通讯作者：
Reiter LT
中科院分区：
文献类型：
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作者：
Goorha S;Reiter LT
研究人类神经遗传性疾病，特别是影响神经系统的罕见综合征的一个主要问题是培养准确代表这些疾病用于分析的神经元培养物的能力。虽然从皮肤和血液中产生诱导多能干细胞（iPSC）已经取得了一些成功，但从这些生物标本中收集和生产iPSC仍然存在局限性。我们已经在收集和培养人类牙髓干细胞（DPSC）方面取得了重大成功，这些干细胞来自于患有各种罕见神经遗传综合征的儿童的父母送到我们实验室的脱落牙齿。该方案概述了我们目前用于从脱落（婴儿）牙齿生长和扩增DPSC的方法。这些DPSC可以分化成多种细胞类型，包括成骨细胞、软骨细胞和混合神经元和神经胶质培养物。在这里，我们提供了我们的协议，分化早期传代DPSC培养成神经元的分子研究。
A major issue in studying human neurogenetic disorders, especially rare syndromes affecting the nervous system, is the ability to grow neuronal cultures that accurately represent these disorders for analysis. Although there has been some success in generating induced pluripotent stem cells (iPSC) from both skin and blood, there are still limitations to the collection and production of iPSC from these biospecimens. We have had significant success in collecting and growing human dental pulp stem cells (DPSC) from exfoliated teeth sent to our laboratory by the parents of children with a variety of rare neurogenetic syndromes. This protocol outlines our current methods for the growth and expansion of DPSC from exfoliated (baby) teeth. These DPSC can be differentiated into a variety of cell types including osteoblast, chondrocytes and mixed neuron and glial cultures. Here we provide our protocol for the differentiation of early passage DPSC cultures into neurons for molecular studies.