Measurement of ovine SGLT1 mRNA levels by competitive RT-PCR.
Measurement of ovine SGLT1 mRNA levels by competitive RT-PCR.
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通过竞争性 RT-PCR 测量绵羊 SGLT1 mRNA 水平。
DOI：
10.1042/bst025481s
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发表时间：
1997
影响因子：
3.9
通讯作者：
S. Shirazi
中科院分区：
文献类型：
--
作者：
G. Allison;S. Shirazi
但不改变mRNA表达水平。D-葡萄糖直接注入nunkunt dream的iDdCs中，可使SGLT 1转运活性和蛋白丰度增加60-90倍，但仅使SGLT 1 mRNA丰度增加2倍。因此，我们得出结论，SGLT 1表达的调节与mRNA丰度无关[4]。进一步的研究表明，在2-3岁反刍羊中，功能性SGLTI蛋白和mRNA的水平比在哺乳前羔羊中测得的水平低500倍以上。向管腔内容物中引入D-葡萄糖和一些葡萄糖类似物，可增强SGLT 1蛋白和mRNA的表达，从而在未断奶羔羊中观察到类似的丰度水平。后者的数据也表明accllularcontmlme和mismatthelweloftmma@ im。为了检测SGLT 1 mRNA在不同糖浓度下的丰度变化，我们建立了一种半定量的、具有竞争力的、具有更强的抗逆转录酶活性的北方印迹法，并可直接测定转录本丰度。我们已经建立了一个
UdtrrmspatacriVity to prarWniaant levels but did not change mRNA abuadsncc. Direct infushofD-gIuwse intothe lumiual oontents ofthe iDdcsoiac of nunkunt dreep imeesed SGLTl transport activity and pmtein abundsnce 60-90 bld, but only inmased SGLTl mRNA abundance 2 fold. We concluded ttrerefore, that the regdation of SGLTl expression is dissociated from mRNA abundance [4]. Further work has shown that in 2-3 year old ruminaat sheep, levels of both functiooal SGLTI protein and mRNA are> 500-fold lower than those mc~ sured in pre-mninant lambs. Introduction of D-glucose and some glucose analogues, to the luminal contents mults in enhancement of expnsSion of both SGLTl protein and mRNA to snnilar levds of abundrnce obsemd in pre-tuminsnb lambs. The latter data also indicatesaccllularcontmlme&mismatthelweloftmma@ im. In &to asses8 changes in the abundance of SGLTl mRNA in~ duriqgdtvtbpmcnt, andwithcbsagcsinthelevelsof~ sugars, we have developed a semiquambtive, oompetifive reverse-nmgdude more sudivetban Northern blotting and permits transcript abundance lwds to be directly ccanpared. We have constructed a