Identification of differentially expressed genes related to metabolic syndrome induced with high-fat diet in E3 rats
Identification of differentially expressed genes related to metabolic syndrome induced with high-fat diet in E3 rats
复制标题
E3大鼠高脂饮食代谢综合征相关差异表达基因的鉴定
DOI：
10.1177/1535370214554531
复制
发表时间：
2015-02
影响因子：
3.2
通讯作者：
Lu Shemin
中科院分区：
文献类型：
--
作者：
Lan Xi;Li Dongmin;Zhong Bo;Ren Juan;Wang Xuan;Sun Qingzhu;Li Yue;Liu Lee;Liu Li;Lu Shemin
了解代谢综合征（MetS）异常器官中差异表达的基因有助于揭示该疾病的分子机制和识别新的治疗靶点。本研究旨在筛选高脂饮食诱导的代谢综合征（HFD-MetS）E3大鼠肝脏中MetS相关基因的差异表达。采用高脂饲料喂养E3大鼠24周，诱导MetS。采用抑制性消减杂交（SSH）技术对HFD-MetS与对照E3大鼠肝脏差异表达的基因进行鉴定。从正向消减文库中随机挑选20个阳性重组克隆进行测序。通过GenBank数据库中的BLAST分析，确定各cDNA片段的性质。共鉴定出11个注释基因、3个EST和2个新基因片段。实时定量PCR（RT-qPCR）显示，与对照组相比，HFD-MetS E3大鼠肝脏中已知与MetS相关的4个基因（Alb、Pip 4k 2a、Scd 1和Tf）和其他5个未知与MetS相关的基因（Eif 1、Rnase 4、Rps 12、Rup 2和Tmsb 4）的表达显著上调。通过分析这9个基因的表达与血清TG、Tch、HDL-C和LDL-C浓度的相关性，发现TG与5个基因的表达呈显著正相关（Alb、Eif 1、Pip 4k 2a、Rps 12和Tmsb 4x）、Tch和三个基因Rup 2、Scd 1、Tf 3个基因的表达与HDL-C呈显著负相关。本研究为阐明MetS的分子机制提供了重要线索。
Understanding the genes differentially expressing in aberrant organs of metabolic syndrome (MetS) facilitates the uncovering of molecular mechanisms and the identification of novel therapeutic targets for the disease. This study aimed to identify differentially expressed genes related to MetS in livers of E3 rats with high-fat-diet-induced metabolic syndrome (HFD-MetS). E3 rats were fed with high-fat diet for 24 weeks to induce MetS. Then, suppression subtractive hybridization (SSH) technology was used to identify the genes differentially expressed between HFD-MetS and control E3 rat livers. Twenty positive recombinant clones were chosen randomly from forward subtractive library and sent to sequence. BLAST analysis in GenBank database was used to determine the property of each cDNA fragment. In total, 11 annotated genes, 3 ESTs, and 2 novel gene fragments were identified by SSH technology. The expression of four genes (Alb, Pip4k2a, Scd1, and Tf) known to be associated with MetS and other five genes (Eif1, Rnase4, Rps12, Rup2, and Tmsb4) unknown to be relevant to MetS was significantly up-regulated in the livers of HFD-MetS E3 rats compared with control rats using real-time quantitative PCR (RT-qPCR). By analyzing the correlations between the expression of these nine genes and serum concentrations of TG, Tch, HDL-C, and LDL-C, we found that there were significant positive correlations between TG and the expression of five genes (Alb, Eif1, Pip4k2a, Rps12, and Tmsb4x), Tch and three genes (Rnase4, Scd1, and Tmsb4x), and LDL-C and two genes (Rnase4 and Scd1), as well there were significant negative correlations between HDL-C and the expression of three genes (Rup2, Scd1, and Tf). This study provides important clues for unraveling the molecular mechanisms of MetS.
登录
查看更多内容
DOI：
10.1007/978-1-61779-310-3_15
发表时间：
2011-01-01
期刊：
NEUROPEPTIDES: METHODS AND PROTOCOLS
影响因子：
--
作者：
Ghorbel, Mohamed T.;Murphy, David
通讯作者：
Murphy, David
影响因子：
3.7
作者：
Wei C;Kumar S;Kim IK;Gupta S
通讯作者：
Gupta S
影响因子：
5.5
作者：
Wang, Wei;Zhong, Bo;Lu, Shemin
通讯作者：
Lu, Shemin
影响因子：
2.2
作者：
Gao, Qi;Tancredi, Sarah E.;Thompson, Graham J.
通讯作者：
Thompson, Graham J.
影响因子：
4.1
作者：
Li, Dongmin;Wang, Xuan;Lu, Shemin
通讯作者：
Lu, Shemin