Identification of Fasciola species isolated from Egypt based on sequence analysis of genomic (ITS1 and ITS2) and mitochondrial (NDI and COI) gene markers
Identification of Fasciola species isolated from Egypt based on sequence analysis of genomic (ITS1 and ITS2) and mitochondrial (NDI and COI) gene markers
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DOI：
10.1016/j.parint.2010.09.003
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发表时间：
2011-01-01
影响因子：
1.9
通讯作者：
Nakai, Yutaka
中科院分区：
文献类型：
--
作者：
Amer, Said;Dar, Yasser;Nakai, Yutaka
肝片吸虫病对发达国家和发展中国家的农业都有负面影响，而不是对公共卫生构成挑战。本研究对片孢霉进行了鉴定。根据分子参数和精子发生，从不同的最终宿主(水牛、牛和羊)获得。从埃及不同地区屠宰场屠宰的动物的肝脏中收集了91只成年吸虫。对分析的吸虫的显微镜检查显示，精囊(精子)中有许多正常的精子，表明它们具有生精能力。这项研究表明，埃及没有出现孤雌生殖的片形吸虫物种。利用基因组(ITS1和ITS2)和线粒体(NDI和COI)基因标记进行分子分析。16只动物被证实感染了一种片形吸虫，2只动物同时感染了肝片吸虫和大片吸虫。结果表明，绵羊比巨型肝片吸虫更易感染肝片吸虫(8/10)。ITS1和ITS2核糖体区序列分析表明，该吸虫可分为肝吸虫型(47只)和巨型吸虫(42只)3类，2只吸虫同时具有这两种类型的序列，表明同一基因座上存在不同的等位基因。在ITS1(第96位)和ITS2(第416位)都检测到T/C碱基的独特重叠。根据线粒体基因标记(NDI和COI)的结果，将吸虫分为肝片吸虫型和巨型吸虫。在两个标记上都检测到了广泛的序列内多态性。埃及株的NDI和COI序列与数据库中的相关序列相比显示出明显的多样性。(C)2010爱思唯尔爱尔兰有限公司。保留所有权利。
Fascioliasis has a negative impact on the farming industry in both developed and developing countries, rather than a public health challenge. This study was performed to identify Fasciola sp. from different definitive hosts (buffalo, cattle, and sheep) based on the molecular parameters and spermatogenesis. Ninety-one adult flukes were collected from livers of slaughtered animals at abattoirs in different prefectures in Egypt. Microscopic examination of the analyzed flukes showed many normal spermatozoa in the seminal vesicles (spermic), suggesting that they have the ability of spermatogenesis. This study showed that no parthenogenic Fasciola species occurred in Egypt. Molecular analysis was performed utilizing genomic (ITS1 and ITS2) and mitochondrial (NDI and COI) gene markers. Whereas 16 animals proved to have infection with a single Fasciola species, 2 were infected with both F. hepatica and F. gigantica. The results indicated that sheep were prone to F. hepatica (8 out of 10 animals) more than F. gigantica infection. Sequences of ITS1 and ITS2 ribosomal region indicated that the flukes were categorized into 3 groups F. hepatica-type (47), F. gigantica-type (42) and 2 flukes possessed sequences of both types indicating an existence of different alleles at the same loci. Unique overlapping of T/C bases were detected in both ITS1 (Position 96) and ITS2 (Position 416). Based on results of mitochondrial gene markers (NDI and COI), flukes were classified into F. hepatica-type and F. gigantica-type. Extensive intra-sequence polymorphism was detected at both markers. NDI and COI sequences of Egyptian strain of F. gigantica showed pronounced diversity compared with relevant sequences at database. (C) 2010 Elsevier Ireland Ltd. All rights reserved.