Highly Carboxylated, Cellular Structured, and Underwater Superelastic Nanofibrous Aerogels for Efficient Protein Separation
Highly Carboxylated, Cellular Structured, and Underwater Superelastic Nanofibrous Aerogels for Efficient Protein Separation
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用于高效蛋白质分离的高度羧化、细胞结构和水下超弹性纳米纤维气凝胶
DOI：
10.1002/adfm.201808234
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发表时间：
2019-03-28
影响因子：
19
通讯作者：
Ding, Bin
中科院分区：
文献类型：
--
作者：
Fu, Qiuxia;Si, Yang;Ding, Bin
同时具有大的蛋白质吸附容量和高处理通量的色谱介质在蛋白质分离中是非常必要的，然而，这种材料的创建仍然面临巨大的挑战。本文报道了一种通过结合纳米纤维气凝胶的形成技术和原位改性方法来开发高度羧化的整体介质的稳健策略。得到的离子交换纳米纤维气凝胶(IENFA)具有独特的细胞结构，由柔性的陶瓷纳米纤维和功能聚合物包裹层组成，使其具有优异的水下超弹性和抗压缩疲劳性能(1000次压缩循环后几乎没有塑性变形)。由于IENFA具有相互连接的纳米纤维细胞结构、良好的亲水性、高羧化度和优异的力学性能，使其具有同步提高的静态(2.9×10 3 mg g−1)和动态(1.7×10 3 mg g−1)溶菌酶的吸附能力和改善的缓冲通量(2.17×10 4 L m−2 h−1，重力驱动)，优于已报道的纳米纤维材料和商用离子交换膜。IENFA还具有出色的性能稳定性、易操作和良好的可再生性。此外，IENFA填充柱仅靠重力就可以直接、连续地将溶菌酶从蛋清中分离出来，突出了其优异的实际应用性能。这项工作可能为设计和开发用于生物分离的下一代高效色谱介质提供一条新的途径。
Chromatographic media with synchronously large protein adsorption capacity and high processing flux are highly desired in protein separation; however, the creation of such materials still faces enormous challenges. Herein, a robust strategy to develop highly carboxylated monolithic media by combining nanofibrous aerogels' forming technique and an in situ modification approach is reported. The obtained ion‐exchange nanofibrous aerogels (IENFAs) exhibit a unique cellular structure consisting of flexible ceramic nanofibers and a functional polymer wrapping layer, endowing them with outstanding underwater superelasticity and compressive fatigue resistance (nearly no plastic deformation after 1000 compressive cycles). Benefiting from the interconnected nanofibrous cellular structure, good hydrophilicity, high carboxylation, and excellent mechanical properties, the IENFAs exhibit synchronously promoted static (2.9 × 103 mg g−1) and dynamic (1.7 × 103 mg g−1) lysozyme adsorption capacities and improved buffer flux (2.17 × 104 L m−2 h−1, gravity driven), which are superior to these reported nanofibrous materials and commercial ion‐exchange membranes. The IENFAs also possess outstanding performance stability, easy operation, and excellent regenerability. Moreover, the IENFA‐packed column could directly and continuously separate lysozyme from egg white solely driven by gravity, highlighting their excellent practical application performance. This work may provide a new avenue to design and develop next‐generation high‐performance chromatographic media for bioseparation.