Monoclonal Antibody Specific for Dhx9/NDHII/RHA
Monoclonal Antibody Specific for Dhx9/NDHII/RHA
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DOI：
10.1089/hyb.2009.0107
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发表时间：
2010-06-01
期刊：
影响因子：
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通讯作者：
Ohkawa, Yasuyuki
中科院分区：
文献类型：
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作者：
Kotani, Manato;Harada, Akihito;Ohkawa, Yasuyuki
Dhx9/NDHII/RHA是DEAH家族中的一员，具有一个双链RNA结合域(DsRBD)和一个解旋酶结构域。Deah蛋白家族在RNA代谢中起着关键作用。DEAH家族成员的功能是依赖于ATP的RNA解旋酶和转录调控。在本研究中，我们报道了用大鼠内侧髂骨淋巴结法建立了抗Dhx9的单抗。用我们的抗Dhx9抗体进行免疫印迹分析，检测到全长Dhx9。此外，使用我们的抗体进行的免疫细胞化学染色显示了Dhx9的核定位。这一抗Dhx9的单抗将为进一步详细研究Dhx9的表达奠定基础。
Dhx9/NDHII/RHA is a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain. The DEAH protein family plays a critical role in RNA metabolism. DEAH family members function as ATP-dependent RNA helicases and regulation of transcription. In the present study, we report the establishment of a monoclonal antibody specific for Dhx9 using the rat medial iliac lymph node method. Immunoblot analysis using our antibody against Dhx9 detected full-length Dhx9. In addition, immunocytochemical staining using our antibody against Dhx9 revealed the nuclear localization of Dhx9. This monoclonal antibody against Dhx9 will allow for further detailed studies of Dhx9 expression.