Mice Show Circadian Rhythms of Blood Pressure During Each Wake-Sleep State
Mice Show Circadian Rhythms of Blood Pressure During Each Wake-Sleep State
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DOI：
10.3109/07420528.2011.635231
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Zoccoli, Giovanna
中科院分区：
文献类型：
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作者：
Bastianini, Stefano;Silvani, Alessandro;Zoccoli, Giovanna
血压（BP）的每日节律，在活动期具有最大值，携带重要的预后信息。这种节奏在多大程度上取决于行为因素仍然存在争议。小鼠是功能基因组学的首选物种。在小鼠中，觉醒和睡眠的发作不限于特定的每日时段，允许在一天中的每个时间测量每个觉醒-睡眠状态下的BP。本研究的目的是调查是否昼夜节律的血压是在小鼠的每一个清醒-睡眠状态的表现。将具有B6遗传背景的小鼠（n = 26）植入遥测BP传感器和电极以区分唤醒-睡眠状态，并在12：12 h光暗期下饲养时记录。对于每只小鼠，通过在连续的3小时时间箱上取平均值，在每个觉醒-睡眠状态（觉醒、非快速眼动睡眠和快速眼动睡眠）中获得8个BP值。方差分析表明，在每一个清醒-睡眠状态，以及一个显着的清醒-睡眠状态x时间的相互作用的影响显着的时间效应。在恒定黑暗中记录的另外一组小鼠（n = 3）中，Lomb-Scargle周期图也显示了每个清醒-睡眠状态下BP的显著昼夜节律。这些研究结果表明，在每个清醒-睡眠状态，小鼠显示每日和昼夜节律的BP夹带的条件下的光-暗周期和自由运行的条件下，恒定的黑暗，分别。（作者通讯：giovanna. unibo.it）
A daily rhythm of blood pressure (BP), with maximum values in the activity period, carries important prognostic information. The extent to which this rhythm depends on behavioral factors remains debated. Mice are the species of choice for functional genomics. In mice, episodes of wakefulness and sleep are not restricted to particular daily periods, allowing BP in each wake-sleep state to be measured at each time of day. The aim of this study was to investigate whether a circadian rhythm of BP is manifest in each wake-sleep state in mice. Mice with B6 genetic background (n = 26) were implanted with a telemetric BP transducer and electrodes to discriminate wake-sleep states and recorded while housed under a 12:12 h light-dark period. For each mouse, 8 values of BP were obtained in each wake-sleep state (wakefulness, non-rapid-eye-movement sleep, and rapid-eye-movement sleep) by averaging over successive 3-h time bins. Analysis of variance evidenced a significant time effect in each wake-sleep state as well as a significant wake-sleep state x time interaction effect. In an additional group of mice (n = 3) recorded in constant darkness, the Lomb-Scargle periodogram also revealed a significant circadian rhythm of BP in each wake-sleep state. These findings demonstrate that during each wake-sleep state, mice show daily and circadian rhythms of BP in conditions of entrainment to the light-dark cycle and in free-running conditions of constant darkness, respectively. (Author correspondence: giovanna.zoccoli@unibo.it)