Gold cleaning methods for preparation of cell culture surfaces for self-assembled monolayers of zwitterionic oligopeptides
Gold cleaning methods for preparation of cell culture surfaces for self-assembled monolayers of zwitterionic oligopeptides
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DOI：
10.1016/j.jbiosc.2017.12.014
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发表时间：
2018-05-01
影响因子：
2.8
通讯作者：
Fukuda, Junji
中科院分区：
文献类型：
--
作者：
Enomoto, Junko;Kageyama, Tatsuto;Fukuda, Junji
自组装单分子膜（SAM）已被用于阐明细胞和材料表面化学之间的相互作用。用寡肽自组装膜修饰的金表面表现出几个独特的特性，例如细胞排斥表面、用于控制细胞微环境的细胞粘附和非粘附区域的微图案以及在培养条件下在外部刺激时细胞的动态释放。然而，制备寡肽自组装膜的基本程序，包括改性前金表面的适当清洁方法，尚未完全建立。由于金表面很容易被空气中的有机化合物污染，因此清洁方法对于SAM的形成至关重要。在这项研究中，我们研究了四种黄金清洗方法的效果：稀王水，臭氧水，大气等离子体和紫外线照射。在这些方法中，紫外线照射最显着地改善了寡肽自组装膜的形成方面的排斥细胞的表面上。我们制作了一个装置，紫外光源，旋转台，和HEPA过滤器，同时处理多个金基板。此外，紫外线清洁的金基板能够脱离细胞片，而没有严重的细胞损伤。这可能为基于寡肽SAM的生物医学研究实验提供稳定和稳健的方法。(C)2017年，日本生物技术学会。All rights reserved.
Self-assembled monolayers (SAMs) have been used to elucidate interactions between cells and material surface chemistry. Gold surfaces modified with oligopeptide SAMs exhibit several unique characteristics, such as cell-repulsive surfaces, micropatterns of cell adhesion and non-adhesion regions for control over cell microenvironments, and dynamic release of cells upon external stimuli under culture conditions. However, basic procedures for the preparation of oligopeptide SAMs, including appropriate cleaning methods of the gold surface before modification, have not been fully established. Because gold surfaces are readily contaminated with organic compounds in the air, cleaning methods may be critical for SAM formation. In this study, we examined the effects of four gold cleaning methods: dilute aqua regia, an ozone water, atmospheric plasma, and UV irradiation. Among the methods, UV irradiation most significantly improved the formation of oligopeptide SAMs in terms of repulsion of cells on the surfaces. We fabricated an apparatus with a UV light source, a rotation table, and HEPA filter, to treat a number of gold substrates simultaneously. Furthermore, UV-cleaned gold substrates were capable of detaching cell sheets without serious cell injury. This may potentially provide a stable and robust approach to oligopeptide SAM-based experiments for biomedical studies. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.