Emulsified isoflurane preconditioning protects against liver and lung injury in rat model of hemorrhagic shock.
Emulsified isoflurane preconditioning protects against liver and lung injury in rat model of hemorrhagic shock.
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DOI：
10.1016/j.jss.2010.06.037
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发表时间：
2011-12
期刊：
影响因子：
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通讯作者：
Lei Zhang;Nanfu Luo;Jin Liu;Zeyan Duan;G. Du;Jian Cheng;Haixia Lin;Zhuo Li
中科院分区：
文献类型：
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作者：
Lei Zhang;Nanfu Luo;Jin Liu;Zeyan Duan;G. Du;Jian Cheng;Haixia Lin;Zhuo Li
研究背景异氟烷对某些器官的缺血/再灌注损伤具有保护作用。方法将SD大鼠随机分为对照组、失血性休克（HS）组、异氟醚（Iso）组、异氟醚（IL）组和乳化异氟醚（E-Iso）组。注射生理盐水、异氟醚、异氟醚或乳化异氟醚15分钟以上。注射后45分钟，实验组开始出血。复苏后4 h测定丙氨酸氨基转移酶（ALT）、支气管肺泡灌洗液（BAL）中蛋白和细胞含量，并进行肝、肺组织病理学检查。测定肝、肺线粒体丙二醛（MDA）和超氧化物歧化酶（SOD）含量。在失血性休克大鼠中也观察到了存活率，MTT乳化异氟醚提高了存活率，降低了BAL中ALT、蛋白质和细胞含量、肝和肺细胞凋亡以及组织学评分。降低线粒体MDA含量，提高SOD活性。IL组肝线粒体SOD活性升高，ALT、肝细胞凋亡及组织学评分降低。结论乳化异氟醚预处理对失血性休克大鼠的肝、肺损伤具有保护作用，并能提高失血性休克大鼠的存活率。其机制可能是抑制细胞死亡和提高线粒体的抗氧化能力。
BACKGROUNDIsoflurane has demonstrated protective effects against ischemia/reperfusion injury in some organs. In this study, using the hemorrhagic shock model, we investigated whether emulsified isoflurane preconditioning protected against liver and lung injury caused by massive surgical blood loss.METHODSMale Sprague-Dawley (SD) rats were randomly divided into five groups: a control group, a hemorrhagic shock (HS) group, an intralipid (IL) group, an isoflurane (Iso) group, and an emulsified isoflurane (E-Iso) group. Saline, intralipid, isoflurane, or emulsified isoflurane were administered over 15 min. Forty-five min after injection, hemorrhage was initiated in the experimental group. Four h after resuscitation alanine aminotransferase (ALT), protein and cellular content in bronchoalveolar lavage fluid (BAL), and the liver and lung histopathology were measured. The malondialdehyde (MDA) and superoxide dismutase (SOD) in the liver and lung mitochondria were tested. The survival was also observed in hemorrhagic shocked rats.RESULTSEmulsified isoflurane enhanced survival and decreased ALT, protein, and cellular content in BAL, liver, and lung apoptosis, and the histologic score. It also decreased MDA and increased SOD activity in mitochondria. In the IL group, liver mitochondrial SOD activity increased, while ALT, liver apoptosis and histological score decreased. In the Iso group liver and lung mitochondrial SOD activity increased, while liver and lung apoptosis decreased.CONCLUSIONEmulsified isoflurane preconditioning has a protective effect against liver and lung injury as well as improving the survival in hemorrhagic shock. The potential mechanisms involved are the inhibition of cell death and improvement of antioxidation in mitochondria.