Identification of the cis-acting elements in Arabidopsis thaliana NHL10 promoter responsible for leaf senescence, the hypersensitive response against Cucumber mosaic virus infection, and spermine treatment
Identification of the cis-acting elements in Arabidopsis thaliana NHL10 promoter responsible for leaf senescence, the hypersensitive response against Cucumber mosaic virus infection, and spermine treatment
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DOI：
10.1016/j.plantsci.2004.09.016
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发表时间：
2005-02
期刊：
影响因子：
5.2
通讯作者：
Mingqiang Zheng;Hideki Takahashi;A. Miyazaki;K. Yamaguchi;T. Kusano
中科院分区：
文献类型：
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作者：
Mingqiang Zheng;Hideki Takahashi;A. Miyazaki;K. Yamaguchi;T. Kusano
拟南芥中有45个NDR 1/HIN 1-like（NHL）基因。其中，我们关注NHL 10基因，因为其独特的表达谱：在用无毒黄瓜花叶病毒（CMV）株引发的过敏反应（HR）期间，以及通过用多胺精胺（Spm [M. S.郑惠南Takahashi，A.宫崎，H。Hamamoto，J. Shah，I.山口T. Kusano，Up-regulation of Arabidopsis thalianaNHL10 in the hypersensitive response to Cucumber mosaic virus infection and in senescing leaves is controlled by signaling pathways that different in salicylate involvement，Planta 218（2004）740-750]）。我们利用β-葡萄糖醛酸酶（GUS）报告基因融合构建体在转基因拟南芥中对NHL 10启动子进行了分析。拟南芥植物。通过组织化学染色分析，证明了NHL 10启动子的假定转录起始位点的-697和+53之间的区域对于叶片衰老、对CMV的HR和Spm处理的响应是必需的。这些数据通过使用GUS报告基因作为探针的北方分析得到证实。通过进一步的缺失分析，所有对叶片衰老、CMV诱导的HR和Spm处理有反应的顺式作用元件都被缩小到相同的-198到-93区域。NHL 10基因的表达在转录水平上受到调控。
There are 45 NDR1/HIN1-like (NHL) genes in Arabidopsis thaliana. Of these, we have focused on the NHL10 gene because of its unique expression profiles: its transcripts are accumulated in a senescence-associated manner, during the hypersensitive response (HR) triggered with an avirulent Cucumber mosaic virus (CMV) strain, and by treatment with the polyamine spermine (Spm [M.S. Zheng, H. Takahashi, A. Miyazaki, H. Hamamoto, J. Shah, I. Yamaguchi, T. Kusano, Up-regulation of Arabidopsis thalianaNHL10 in the hypersensitive response to Cucumber mosaic virus infection and in senescing leaves is controlled by signalling pathways that differ in salicylate involvement, Planta 218 (2004) 740–750]). Here we have performed an analysis of NHL10 promoter using the β-glucuronidase (GUS) reporter gene-fusion constructs in transgenic A. thaliana plants. With the histochemical staining assay, it was demonstrated that the region between −697 and +53 from the putative transcriptional start site of the NHL10 promoter was essential for responses to leaf senescence, HR to CMV and Spm treatment. These data were confirmed by northern analysis using the GUS reporter gene as a probe. By further deletion analysis, all the cis-acting elements responsive to leaf senescence, CMV-induced HR, and Spm treatment, were narrowed down to the identical −198 to −93 region. It also demonstrated that the controlled expression of NHL10 gene at a transcriptional level.