A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening
A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening
复制标题
番茄液泡转化酶抑制剂介导蔗糖代谢并影响果实成熟
DOI：
10.1104/pp.16.01269
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发表时间：
2016-11-01
期刊：
影响因子：
7.4
通讯作者：
Tian, Shiping
中科院分区：
文献类型：
--
作者：
Qin, Guozheng;Zhu, Zhu;Tian, Shiping
果实成熟是一个复杂的过程，涉及一系列生理生化变化，最终影响果实的品质性状，如颜色和风味。糖代谢是成熟的一个重要因素，有证据表明，它影响成熟的各个方面，尽管相关的机制还没有很好地理解。在这项研究中，我们确定和分析了36个基因的表达参与Suc代谢成熟番茄（Solanum lycopersicum）果实。染色质免疫沉淀和凝胶迁移率变动分析表明，SIVIF，它编码的液泡转化酶抑制剂，和SIVI，编码的液泡转化酶，直接由全球水果成熟调节RIPENING抑制剂（RIN）。此外，我们表明，SlVIF物理相互作用与SlVI控制Suc代谢。RNA干扰抑制SlVIF延迟番茄果实成熟，而过表达SlVIF加速成熟，伴随着番茄红素的产生和乙烯生物合成的变化。基于相对和绝对定量的定量蛋白质组学分析的同量异序标签进一步表明，通过抑制SlVIF表达，改变了一组参与果实成熟的蛋白质的丰度，包括与番茄红素生成和乙烯合成相关的蛋白质。这些发现为Suc在促进果实成熟中的作用提供了证据，并确立了SlVIF有助于果实品质和RIN介导的成熟调节机制，这具有重要的农业价值。
Fruit ripening is a complex process that involves a series of physiological and biochemical changes that ultimately influence fruit quality traits, such as color and flavor. Sugar metabolism is an important factor in ripening, and there is evidence that it influences various aspects of ripening, although the associated mechanism is not well understood. In this study, we identified and analyzed the expression of 36 genes involved in Suc metabolism in ripening tomato (Solanum lycopersicum) fruit. Chromatin immunoprecipitation and gel mobility shift assays indicated that SlVIF, which encodes a vacuolar invertase inhibitor, and SlVI, encoding a vacuolar invertase, are directly regulated by the global fruit ripening regulator RIPENING INHIBITOR (RIN). Moreover, we showed that SlVIF physically interacts with SlVI to control Suc metabolism. Repression of SlVIF by RNA interference delayed tomato fruit ripening, while overexpression of SlVIF accelerated ripening, with concomitant changes in lycopene production and ethylene biosynthesis. An isobaric tags for relative and absolute quantification-based quantitative proteomic analysis further indicated that the abundance of a set of proteins involved in fruit ripening was altered by suppressing SlVIF expression, including proteins associated with lycopene generation and ethylene synthesis. These findings provide evidence for the role of Suc in promoting fruit ripening and establish that SlVIF contributes to fruit quality and the RIN-mediated ripening regulatory mechanisms, which are of significant agricultural value.