Transcriptomics and proteomics analysis to explore the mechanism of Yishen Tongluo formula repairing sperm DNA damage in rats
Transcriptomics and proteomics analysis to explore the mechanism of Yishen Tongluo formula repairing sperm DNA damage in rats
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DOI：
10.1111/and.14582
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发表时间：
2022-09
期刊：
影响因子：
2.4
通讯作者：
Chenming Zhang;Jianshe Chen;Xiao Wei;Lina Zhao;Pei-Ge Zhao;Xun Li;Jianwei Cui;Sicheng Ma;Zixue Sun;Zulong Wang
中科院分区：
文献类型：
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作者：
Chenming Zhang;Jianshe Chen;Xiao Wei;Lina Zhao;Pei-Ge Zhao;Xun Li;Jianwei Cui;Sicheng Ma;Zixue Sun;Zulong Wang
精子DNA断裂指数（DFI）是衡量男性生育能力的客观指标。目前，针对高精子DFI的有效治疗方法有限，中药在这方面具有一定的优势。益肾通络方（YSTL）是一种中药方剂，具有降低患者DFI的作用。为了更好地了解其活性机制，我们利用转录组学和蛋白质组学分析了潜在的靶基因YSTL修复雷雷藤苷（TGs）介导的大鼠精子DNA损伤，随后使用RT - qPCR和western blotting进行验证分析，结果表明，相对于对照组，TGs组的DFI明显升高，但相对于TGs组，YSTL组的DFI明显降低。通过趋势分析对119个差异表达基因和158个DEPs进行KEGG通路分析，发现它们在转录组水平上富集于细胞凋亡和碱基切除修复，在蛋白质组水平上富集于癌症、补体和凝血级联反应中的microrna。Ttr和Pnpla2被确定为潜在的YSTL靶基因。我们的数据表明，YSTL可以保护大鼠精子DNA免受TGs诱导的损伤，其可能与细胞凋亡、DNA修复等途径有关，可能的靶基因是Ttr和Pnpla2。
The sperm DNA fragmentation index (DFI) is an objective indicator of male fertility. Currently, effective treatments for high sperm DFI are limited and traditional Chinese medicine (TCM) has certain advantages in this aspect. Yishen Tongluo formula (YSTL), a TCM formula, has been found to reduce DFI in patients. To better understand the mechanisms underlying its activity, we used transcriptomics and proteomics to analyse the potential target gene YSTL repairing tripterygium glycosides (TGs)‐mediated sperm DNA damage in rats, followed by validation analyses using RT‐qPCR and western blotting, which showed that relative to the control group, DFI was markedly elevated in the TGs group, but markedly lower in the YSTL group relative to the TGs group. KEGG pathway analysis of 119 differentially expressed genes and 158 DEPs identified using trend analysis revealed that they were enriched for apoptosis and base excision repair at the transcriptomic level and for microRNAs in cancer and complement and coagulation cascades at the proteomic level. Ttr and Pnpla2 were identified as potential target genes for YSTL. Our data show that YSTL can protect rat sperm DNA from TGs‐induced damage, which may be related to apoptosis, DNA repair and other pathways, and the possible target genes are Ttr and Pnpla2.