Effect of thymoquinone on Fusobacterium nucleatum-associated biofilm and inflammation
Effect of thymoquinone on Fusobacterium nucleatum-associated biofilm and inflammation
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DOI：
10.3892/mmr.2020.11136
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发表时间：
2020-05
影响因子：
3.4
通讯作者：
Ayano Tada;H. Nakayama-Imaohji;H. Yamasaki;M. Elahi;T. Nagao;Hirofumi Yagi;M. Ishikawa;K. Shibuya;T. Kuwahara
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文献类型：
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作者：
Ayano Tada;H. Nakayama-Imaohji;H. Yamasaki;M. Elahi;T. Nagao;Hirofumi Yagi;M. Ishikawa;K. Shibuya;T. Kuwahara
牙周炎会影响口腔组织并引起全身炎症，从而增加心血管疾病和代谢综合征的风险。龈下菌斑积聚是牙周炎的触发因素。具核梭杆菌（FN）通过在牙齿表面嵌入早期和晚期细菌定殖体而促进龈下生物膜的复杂性。此外，对FN的炎症反应与牙周炎的进展有关。黑种草种子，这是众所周知的黑孜然（公元前），已被用作草药治疗疾病，如哮喘和传染病。目前的研究检查了BC油及其活性成分百里酚（TM）和百里醌（TQ）对FN相关生物膜和炎症的抑制作用。通过与早期牙定殖菌内氏放线菌（Actinomycesnaeslundii，AN）共培养制备含FN的生物膜。FN/AN双组分生物膜的稳定性和生物量显著高于单独的FN。这种效果甚至保留与prefixed细胞，表明FN/AN共聚集介导的物理化学相互作用与细胞表面分子。0.1%TM或TQ可显著抑制FN/AN生物膜的形成。共聚焦激光扫描显微镜表明，预成型的FN/AN生物膜与0.01%的BC，TM或TQ的治疗显着降低生物膜厚度，和TQ表现出清洁效果相当于异丙基甲基苯酚。TQ剂量依赖性地抑制暴露于FN的人单核细胞系THP-1产生TNF-α，但对THP-1细胞无毒性。这些结果表明，使用TQ的口腔卫生护理可以减少牙龈组织中FN相关的生物膜和炎症。
Periodontitis affects oral tissues and induces systemic inflammation, which increases the risk of cardiovascular disease and metabolic syndrome. Subgingival plaque accumulation is a trigger of periodontitis. Fusobacterium nucleatum (FN) contributes to subgingival biofilm complexity by intercalating with early and late bacterial colonizers on tooth surfaces. In addition, inflammatory responses to FN are associated with the progression of periodontitis. Nigella sativa Lin. seed, which is known as black cumin (BC), has been used as a herbal medicine to treat ailments such as asthma and infectious diseases. The current study examined the inhibitory effect of BC oil and its active constituents, thymol (TM) and thymoquinone (TQ), on FN-associated biofilm and inflammation. FN-containing biofilms were prepared by co-cultivation with an early dental colonizer, Actinomyces naeslundii (AN). The stability and biomass of FN/AN dual species biofilms were significantly higher compared with FN alone. This effect was retained even with prefixed cells, indicating that FN/AN co-aggregation is mediated by physicochemical interactions with cell surface molecules. FN/AN biofilm formation was significantly inhibited by 0.1% TM or TQ. Confocal laser scanning microscopy indicated that treatment of preformed FN/AN biofilm with 0.01% of BC, TM or TQ significantly reduced biofilm thickness, and TQ demonstrated a cleansing effect equivalent to that of isopropyl methylphenol. TQ dose-dependently suppressed TNF-α production from a human monocytic cell line, THP-1 exposed to FN, yet showed no toxicity to THP-1 cells. These results indicated that oral hygiene care using TQ could reduce FN-associated biofilm and inflammation in gingival tissue.