Overexpression of sineoculis homeobox homolog 1 predicts poor prognosis of hepatocellular carcinoma.
Overexpression of sineoculis homeobox homolog 1 predicts poor prognosis of hepatocellular carcinoma.
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发表时间：
2014-05
影响因子：
1.4
通讯作者：
Jienan Kong;Xianchun Zhou;Shusen Liu;Tiefeng Jin;Yingshi Piao;Chao Liu;Zhenhua Lin
中科院分区：
文献类型：
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作者：
Jienan Kong;Xianchun Zhou;Shusen Liu;Tiefeng Jin;Yingshi Piao;Chao Liu;Zhenhua Lin
人类Sineoculis同源框同源物1（SIX 1）基因的高表达水平与许多人类恶性肿瘤相关。SIX 1蛋白参与染色质重构和基因转录，在细胞凋亡中起重要作用。本研究探讨了SIX 1在肿瘤进展和肝细胞癌（HCC）预后评估中的作用。采用实时荧光定量PCR、免疫印迹、免疫荧光（IF）和免疫组化（IHC）检测SIX 1在HCC细胞系/组织中的表达，并与癌旁组织和正常肝组织进行比较。统计分析SIX 1过表达与肝癌临床病理特征的关系。采用Kaplan-Meier法计算生存率，采用考克斯比例风险模型分析预后因素与患者生存率之间的关系。SIX 1蛋白在肝癌组织中的阳性表达率为80.9%，显著高于癌旁组织和正常肝组织（P < 0.01）。SIX 1过表达与肿瘤大小、pTNM分期、静脉浸润呈正相关。SIX 1高表达患者的5年生存率明显低于SIX 1低表达患者。多因素分析提示pTNM分期和SIX 1蛋白表达是影响肝癌患者生存的独立危险因素。总之，SIX 1在HCC的进展中起重要作用。SIX 1的高表达是HCC预后不良的独立因素。
High expression levels of the human sineoculis homeobox homolog 1 (SIX1) gene have been correlated with numerous human malignancies. The SIX1 protein is involved in chromatin reconstruction and gene transcription, and plays an important role in cell apoptosis. This study explores the role of SIX1 in tumor progression and in the prognostic evaluation of hepatocellular carcinoma (HCC). Real-time PCR, Western blotting analysis, immunofluorescence (IF) staining, and immunohistochemistry (IHC) were performed to examine SIX1 expression in HCC cell line/tissues compared with adjacent non-tumor and normal liver tissues. Statistical analysis was applied to evaluate the correlation between SIX1 overexpression and the clinicopathological features of HCC. Survival rates were calculated using the Kaplan-Meier method, and the relationship between prognostic factors and patient survival was analyzed using the Cox proportional hazard models. The SIX1 protein was detected in 80.9% of HCCs, which was significantly higher than that in either adjacent non tumor liver or normal liver tissues (P < 0.01). SIX1 overexpression was positively correlated with tumor size, pTNM stage and venous infiltration. Moreover, the 5-year survival rate of patients with high expression of SIX1 was significantly lower than that of patients with low SIX1 expression. Multivariate analysis suggested that pTNM stage and SIX1 protein expression were independent risk factors for survival in HCC. In conclusion, SIX1 plays an important role in the progression of HCC. High level expression of SIX1 is an independent poor prognostic factor of HCC.