Nonmuscle myosin II-B (myh10) expression analysis during zebrafish embryonic development
Nonmuscle myosin II-B (myh10) expression analysis during zebrafish embryonic development
复制标题
斑马鱼胚胎发育过程中非肌肉肌球蛋白 II-B (myh10) 的表达分析
DOI：
10.1016/j.gep.2013.04.005
复制
发表时间：
2013-10-01
影响因子：
1.2
通讯作者：
Liu, Dong
中科院分区：
文献类型：
--
作者：
Huang, Yun;Wang, Xueqian;Liu, Dong
非肌肉肌球蛋白II(NM II)是编码不同的非肌肉肌球蛋白重链的三个基因(Myh9、myh10和myh14)的多亚基蛋白产物。这三种NM II亚型具有非常相似的分子结构，在各种基本的生物学过程中发挥着重要的作用。NM II-B(Myh10)对小鼠神经系统和心脏的形成是必不可少的。但到目前为止，对其在斑马鱼胚胎发育过程中的表达还缺乏完整的了解。在本研究中，我们通过In Silicon分析证明了斑马鱼NM II-B在脊椎动物进化中的保守性。之后，NM II-B(Myh10)在原肠胚期开始表达。在20hpF时，其表达主要局限于中枢神经系统。在36hpf及以后，它被维持并扩展到感觉器官，包括眼睛、耳囊和嗅球。我们还检测到了48HPF斑马鱼心脏中的myh10mRNA杂交信号。此外，我们还研究了myh9a和myh9b基因在斑马鱼胚胎发育中的分布。结果表明，myh10和myh9有不同的表达模式，myh9不在神经系统，而是在表皮的包膜层(EVL)。我们的研究为NM II的表达提供了新的见解，并利用这一模式生物来解决未来关于NM II在胚胎发育中的作用的研究。(C)2013爱思唯尔B.V.保留所有权利。
Nonmuscle myosin II (NM II) is the name given to the multi-subunit protein product of three genes (myh9, myh10, and myh14) encoding different nonmuscle myosin heavy chains. The three NM II isoforms share a very similar molecular structure and play important roles in a variety of fundamental biological processes. NM II-B (myh10) has been shown to be essential for the formation of mouse neural system and heart. But so far the complete knowledge for its expression in developing zebrafish embryos is lacking. In current study, we proved the conservation of zebrafish NM II-B in vertebrate evolution by in silicon analysis. Afterwards the NM II-B (myh10) expression was demonstrated to initiate after gastrulation stage. At 20 hpf, the expression is mainly restricted in central nervous system (CNS). It was maintained and expanded to sensor organ including eye, otic vesicle, and olfactory bulb at 36 hpf and later. We also detected myh10 mRNA hybridization signal in 48 hpf zebrafish heart. In addition, we investigated myh9a and myh9b mRNA distribution in zebrafish developing embryos. It was shown that myh10 and myh9 have distinct expression pattern, with myh9s not in neural system but in epidermis, enveloping layer (EVL). Our study provides new insight into the NM II expression and the use of this model organism to tackle future studies on the role of NM II in embryo development. (c) 2013 Elsevier B.V. All rights reserved.