The Pseudomonas aeruginosa lectin LecB binds to the exopolysaccharide Psl and stabilizes the biofilm matrix
The Pseudomonas aeruginosa lectin LecB binds to the exopolysaccharide Psl and stabilizes the biofilm matrix
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DOI：
10.1038/s41467-019-10201-4
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发表时间：
2019-05-16
影响因子：
16.6
通讯作者：
Parsek, Matthew R.
中科院分区：
文献类型：
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作者：
da Silva, Daniel Passos;Matwichuk, Michael L.;Parsek, Matthew R.
铜绿假单胞菌生物膜由胞外多糖 (EPS)、外源 DNA 和将这些群落结合在一起的蛋白质组成。铜绿假单胞菌产生凝集素 LecA 和 LecB，它们对基质 EPS 中的糖具有亲和力，并介导铜绿假单胞菌对靶宿主细胞的粘附。在这里，我们证明 LecB 与 Psl（EPS 的关键基质）结合，这导致细胞和 EPS 在生长的生物膜中的保留增加。预计这种相互作用发生在凝集素和 Psl 上存在的支链侧链之间。最后，我们证明 LecB 协调生物膜中的 Psl 定位。这构成了 LecB 的独特功能，并将其识别为一种基质蛋白，通过 EPS 相互作用促进生物膜结构。
Pseudomonas aeruginosa biofilms are composed of exopolysaccharides (EPS), exogenous DNA, and proteins that hold these communities together. P. aeruginosa produces lectins LecA and LecB, which possess affinities towards sugars found in matrix EPS and mediate adherence of P. aeruginosa to target host cells. Here, we demonstrate that LecB binds to Psl, a key matrix EPS, and this leads to increased retention of both cells and EPS in a growing biofilm. This interaction is predicted to occur between the lectin and the branched side chains present on Psl. Finally, we show that LecB coordinates Psl localization in the biofilm. This constitutes a unique function for LecB and identifies it as a matrix protein that contributes to biofilm structure through EPS interactions.