Presence and Antimicrobial Susceptibility of RE-cmeABC-Positive Campylobacter Isolated from Food-Producing Animals, 2014-2016
Presence and Antimicrobial Susceptibility of RE-cmeABC-Positive Campylobacter Isolated from Food-Producing Animals, 2014-2016
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2014-2016 年从食品生产动物中分离出的 RE-cmeABC 阳性弯曲杆菌的存在和抗菌药物敏感性
DOI：
10.1016/j.eng.2019.10.013
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发表时间：
2020
期刊：
影响因子：
12.8
通讯作者：
Shen Jianzhong
中科院分区：
文献类型：
--
作者：
Liu Dejun;Liu Weiwen;Li Xing;Yao Hong;Shen Zhangqi;Wang Yang;Shen Jianzhong
Campylobacterspp. are the leading cause of human gastroenteritis worldwide. RE-CmeABC is a newly identified resistance-enhancing multidrug efflux pump ofCampylobacterspp. (C.spp.) that confers high-level resistance to fluoroquinolones, phenicols, macrolides, and tetracyclines (TETs), all of which are critical drugs in both human and veterinary medicine. In this study, we analyzed the presence and antimicrobial susceptibility of RE-cmeABC-positiveCampylobacterisolates of food-animal origin from three representative regions (Shandong, Shanghai, and Guangdong) in China over three successive years, from 2014 to 2016. A total of 1088Campylobacterisolates (931C. coliand 157C. jejuni) were recovered from the RE-cmeABCscreening. We detected 122 (11.2%) RE-cmeABC-positive isolates of chicken origin, including 111 (70.7%)C. jejuniand 11 (1.2%)C. coli. This multidrug efflux pump is more prevalent amongC. jejunithanC. coli. The level of resistance was significantly different in 111 RE-cmeABC-positiveC. jejuniversus 46 RE-cmeABC-negativeC. jejunifor florfenicol, clindamycin, and erythromycin (P< 0.05), but not for ciprofloxacin (CIP), TET, and gentamicin (GEN). However, the isolates harboring RE-cmeABCcould shift the minimum inhibitory concentration distribution to the higher range for CIP and TET. Pulsed-field gel electrophoresis (PFGE) analysis suggested that horizontal transmission might be involved in the dissemination of RE-cmeABCin Shanghai and Guangdong, while clonal expansion was predominant in Shandong. Three isolates shared the indiscriminate PFGE types of RE-cmeABC-positiveC. jejuniisolates in Shanghai and Guangdong, and four isolates in Shanghai and Shandong. Our study suggests the possibility of a wide dissemination of RE-cmeABCinCampylobacterof food-animal origin, which would pose a significant threat to public health.
