The developmental regulator PatD modulates assembly of the cell-division protein FtsZ in the cyanobacterium Anabaena sp. PCC 7120.
The developmental regulator PatD modulates assembly of the cell-division protein FtsZ in the cyanobacterium Anabaena sp. PCC 7120.
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发育调节因子 PatD 调节蓝藻鱼腥藻中细胞分裂蛋白 FtsZ 的组装。
DOI：
10.1111/1462-2920.15682
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发表时间：
2021
影响因子：
5.1
通讯作者：
Cheng‐Cai Zhang
中科院分区：
文献类型：
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作者：
Li Wang;Tian;A. Valladares;G. Lin;Ju;A. Herrero;Wenli Chen;Cheng‐Cai Zhang
FtsZ是一种微管蛋白样GTdR，其聚合以启动细菌中的细胞分裂过程。异形胞是丝状蓝藻的终末分化细胞，其已经失去细胞分裂能力，并且其中ftsZ基因被下调。然而，在异形胞分化过程中FtsZ调控的机制却很少被研究。patD基因是NtcA依赖性的，并参与鱼腥藻PCC 7120中异形胞频率的优化。在这里，我们报告说，patD的失活导致形成多个FtsZ环的营养细胞，细胞扩大，并保留在异形胞的肽聚糖合成活性，而其异位表达导致异常FtsZ聚合和细胞分裂。PatD与FtsZ相互作用，增加FtsZ沉淀在沉降试验中，并促进形成厚直FtsZ束，不同于单独由FtsZ形成的环形聚集体。这些结果表明，在分化的异形胞，PatD干扰FtsZ的组装。我们建议，鱼腥藻FtsZ是一个双功能蛋白参与营养细胞分裂和异形胞分化的调节。在分化细胞中，PatD-FtsZ相互作用似乎设定了FtsZ活性，该活性对于细胞分裂是不足的，但对于促进分化是最佳的。本文受版权保护。All rights reserved.
FtsZ is a tubulin-like GTPase that polymerizes to initiate the process of cell division in bacteria. Heterocysts are terminally differentiated cells of filamentous cyanobacteria that have lost the capacity for cell division and in which the ftsZ gene is downregulated. However, mechanisms of FtsZ regulation during heterocyst differentiation have been scarcely investigated. The patD gene is NtcA-dependent and involved in the optimization of heterocyst frequency in Anabaena sp. PCC 7120. Here we report that the inactivation of patD caused the formation of multiple FtsZ-rings in vegetative cells, cell enlargement, and the retention of peptidoglycan synthesis activity in heterocysts, whereas its ectopic expression resulted in aberrant FtsZ polymerization and cell division. PatD interacted with FtsZ, increased FtsZ precipitation in sedimentation assays, and promoted the formation of thick straight FtsZ bundles that differ from the toroidal aggregates formed by FtsZ alone. These results suggest that in the differentiating heterocysts, PatD interferes with the assembly of FtsZ. We propose that in Anabaena FtsZ is a bifunctional protein involved in both vegetative cell division and regulation of heterocyst differentiation. In the differentiating cells PatD-FtsZ interactions appear to set an FtsZ activity that is insufficient for cell division but optimal to foster differentiation. This article is protected by copyright. All rights reserved.