Lancefield group C Streptococcus dysgalactiae infection responsible for fish mortalities in Japan
Lancefield group C Streptococcus dysgalactiae infection responsible for fish mortalities in Japan
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DOI：
10.1111/j.1365-2761.2004.00591.x
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发表时间：
2004-12-01
影响因子：
2.5
通讯作者：
Yoshida, T
中科院分区：
文献类型：
--
作者：
Nomoto, R;Munasinghe, LI;Yoshida, T
从日本用加氏乳球菌商业疫苗免疫的培养的琥珀鱼、杜氏Seriola dumerili Risso和黄尾鱼、五角Seriola quinqueradiata Temminck和Schlegel中分离到一株兰斯菲尔德血清学C群链球菌。分离的细菌为革兰氏阳性球菌，在生理盐水中自聚集，在生长培养基中形态上为长链，过氧化氢酶阴性，在血琼脂上为α-溶血性。测定了两株菌的16 SrDNA的全基因序列，并与数据库中的菌株进行了比较。根据16 S rDNA序列测定、细菌学特性和兰斯菲尔德血清学分型结果，将分离菌株鉴定为停乳链球菌。设计了一对特异于16 S-23 S rDNA基因间隔区的寡核苷酸引物。dysgalactiae从所有的鱼类分离株中扩增了一个基因，以及α-溶血性S.停乳亚种停乳链球菌ATCC 430738和β-溶血性链球菌。停乳亚种equisimilis ATCC 35666，而S.马乳球菌ATCC 33398、格氏乳球菌ATCC 43921和L. Garvieae KG 9408.在实验感染的鱼中看到的尾柄的严重坏死病变与自然感染的鱼中看到的相似。
A Lancefield serological group C Streptococcus sp. was isolated from cultured amberjack, Seriola dumerili Risso, and yellowtail, Seriola quinqueradiata Temminck and Schlegel, immunized with Lactococcus garvieae commercial vaccines in Japan. The isolated bacteria were Gram-positive cocci, auto-aggregating in saline, morphologically long chains in growth medium, catalase negative and alpha-haemolytic on blood agar. An almost complete gene sequence of the 16S rDNA of two isolates was determined and compared with that of bacterial strains in the database. The isolates were identified as Streptococcus dysgalactiae based on the results of the 16S rDNA sequence, the bacteriological properties and the Lancefield serological grouping. Oligonucleotide primers specifically designed for the 16S-23S rDNA intergenic spacer region of S. dysgalactiae amplified a gene from all the fish isolates, as well as the type strains alpha-haemolytic S. dysgalactiae subsp. dysgalactiae ATCC430738 and beta-haemolytic S. dysgalactiae subsp. equisimilis ATCC35666, but not those of S. equi ATCC33398, Lactococcus garvieae ATCC43921 and L. garvieae KG9408. The severe necrotic lesions of the caudal peduncle seen in experimentally infected fish were similar to those seen in naturally infected fish.