A Large Family of Putative Transmembrane Receptors Homologous to the Product of the Drosophila Tissue Polarity Gene frizzled(*)
A Large Family of Putative Transmembrane Receptors Homologous to the Product of the Drosophila Tissue Polarity Gene frizzled(*)
复制标题
DOI：
10.1074/jbc.271.8.4468
复制
发表时间：
1996-02
期刊：
影响因子：
--
通讯作者：
Yanshu Wang;J. Macke;Benjamin S. Abella;Katrin Andreasson;P. Worley;D. Gilbert;N. Copeland;N. Jenkins;J. Nathans
中科院分区：
文献类型：
--
作者：
Yanshu Wang;J. Macke;Benjamin S. Abella;Katrin Andreasson;P. Worley;D. Gilbert;N. Copeland;N. Jenkins;J. Nathans
在果蝇中，卷曲基因在组织极性的发展中起着至关重要的作用，通过表皮结构的方向来评估。通过随机cDNA测序，简并聚合酶链反应扩增和低严格性杂交的组合，我们已经确定了6个新的frizzled同源物从哺乳动物，至少11个从斑马鱼，几个从鸡和海胆，和一个从秀丽隐杆线虫。哺乳动物和线虫同源物的完整推导的氨基酸序列与果蝇frizzled蛋白共享一个保守的氨基末端富含半胱氨酸的结构域和七个推定的跨膜片段。每种哺乳动物同源物在成体的一组独特的组织中表达，并且至少三种在胚胎发生期间表达。作为假设的果蝇卷曲蛋白，卷曲同源物很可能作为跨膜受体尚未确定的配体。这些观察结果预测存在一个家庭的信号转导途径，是同源的途径，决定组织极性在果蝇。
In Drosophila melanogaster, the frizzled gene plays an essential role in the development of tissue polarity as assessed by the orientation of cuticular structures. Through a combination of random cDNA sequencing, degenerate polymerase chain reaction amplification, and low stringency hybridization we have identified six novel frizzled homologues from mammals, at least 11 from zebrafish, several from chicken and sea urchin, and one from Caenorhabditis elegans. The complete deduced amino acid sequences of the mammalian and nematode homologues share with the Drosophila frizzled protein a conserved amino-terminal cysteine-rich domain and seven putative transmembrane segments. Each of the mammalian homologues is expressed in a distinctive set of tissues in the adult, and at least three are expressed during embryogenesis. As hypothesized for the Drosophila frizzled protein, the frizzled homologues are likely to act as transmembrane receptors for as yet unidentified ligands. These observations predict the existence of a family of signal transduction pathways that are homologous to the pathway that determines tissue polarity in Drosophila.